Effect of Gold Nanoparticles on the TLR2-Mediated Inflammatory Responses Induced by Leptospira in TLR2-Overexpressed HEK293 Cells
| Autor: | Rojrit Rojanathanes, Chawikan Boonwong, Kanitha Patarakul, Sasin Thamakaison, Pattama Ekpo, Kanidta Sooklert, Chintana Chirathaworn, Amornpun Sereemaspun |
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| Rok vydání: | 2020 |
| Předmět: |
animal diseases
General Chemical Engineering Inflammation 02 engineering and technology Proinflammatory cytokine lcsh:Chemistry 03 medical and health sciences Immune system Leptospira medicine TLR2 leptospirosis General Materials Science size-dependent 030304 developmental biology 0303 health sciences biology Chemistry Interleukin bacterial infections and mycoses 021001 nanoscience & nanotechnology biology.organism_classification Molecular biology Reverse transcription polymerase chain reaction lcsh:QD1-999 bacteria Tumor necrosis factor alpha medicine.symptom 0210 nano-technology gold nanoparticle |
| Zdroj: | Nanomaterials, Vol 10, Iss 2522, p 2522 (2020) Nanomaterials Volume 10 Issue 12 |
| ISSN: | 2079-4991 |
| DOI: | 10.3390/nano10122522 |
| Popis: | Leptospira infection can cause potential hazards to human health by stimulating inflammation, which is mediated mainly through the Toll-like receptor 2 (TLR2) pathway. Gold nanoparticles (AuNPs) are promising for medical applications, as they display both bioinert and noncytotoxic characteristics. AuNPs have been shown to have the ability to modify immune responses. To understand the in vitro immunomodulatory effect of AuNPs in a Leptospira infection model, the activation of TLR2 expression was examined in HEK-Blue-hTLR2 cells treated with Leptospira serovars and/or AuNPs (10 and 20 nm). The ability of AuNPs to modulate an inflammatory response induced by Leptospira was examined in terms of transcript expression level modulation of three proinflammatory cytokines (tumor necrosis factor-&alpha interleukin (IL)-1&beta and IL-6) using two-stage quantitative real-time reverse transcriptase PCR. The results revealed that the administration of 10 nm AuNPs could augment the Leptospira-induced TLR2 signaling response and upregulate the expression of all three cytokine gene transcripts, whereas the 20 nm AuNPs attenuated the TLR2 activation and expression of proinflammatory cytokines. This indicates that AuNPs can modulate inflammatory parameters in Leptospira infection and different-sized AuNPs had different immunomodulatory functions in this model. |
| Databáze: | OpenAIRE |
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