Application of green-extraction technique to evaluate of antioxidative capacity of wild population of fireweed (Epilobium angustifolium)
Autor: | Katarzyna Florkowska, Wiktoria Duchnik, Anita Siedłowska, Adam Klimowicz, Łukasz Kucharski, Anna Nowak, Kinga Nadarzewska, Daria Wira, Joanna Zielonka-Brzezicka, Anna Muzykiewicz |
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Rok vydání: | 2019 |
Předmět: |
Antioxidant
DPPH medicine.medical_treatment Population Flavonoid antioxidant activity total polyphenol content 01 natural sciences green-extraction technique SB1-1110 chemistry.chemical_compound epilobium angustifolium medicine Food science education chemistry.chemical_classification education.field_of_study ABTS 010405 organic chemistry Plant culture Ripening 0104 chemical sciences total flavonoid content 010404 medicinal & biomolecular chemistry chemistry Polyphenol Weed |
Zdroj: | Herba Polonica, Vol 65, Iss 4, Pp 18-30 (2019) |
ISSN: | 2449-8343 |
DOI: | 10.2478/hepo-2019-0022 |
Popis: | Summary Introduction: Fireweed (Epilobium angustifolium (L.) Holub) is a common weed growing on meadows, roadside and agricultural wasteland, creating vast, rapidly spreading fields. Objective: The aim of the study was to evaluate the antioxidant activity of extracts from fresh fireweed, harvested at three ripening stages. Methods: Analysis of antioxidative activity was carried out by DPPH, ABTS and FRAP methods. Total polyphenol and total flavonoid content were also determined. Plant material was extracted using ultra-sound-assisted green extraction technique with methanol, ethanol and isopropanol at different concentrations and water. Results: The highest antioxidant activity evaluated by DPPH, ABTS and FRAP was found for the extracts prepared in 70% ethanol. The highest content of total polyphenols were observed in extracts in 70% ethanol, whereas the highest content of flavonoids extracts in undiluted methanol. Conclusion: Epilobium angustifolium harvested at fruit ripening stage seems to be a valuable source of antioxidants. |
Databáze: | OpenAIRE |
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