Determination of the reference range of endogenous plasma carnitines in healthy adults
| Autor: | Donald H Chace, Gianfranco Fornasini, Allan M. Evans, Stephanie E. Reuter |
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| Přispěvatelé: | Reuter, Lange Stephanie Elizabeth, Evans, Allan Mark, Chace, Donald, Fornasini, Gianfranco |
| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Spectrometry Mass Electrospray Ionization medicine.medical_specialty Adolescent Electrospray ionization Clinical Biochemistry Reference range Endogeny Young Adult 110302 [FOR] acylcarnitines Reference Values Carnitine Internal medicine medicine Industrial Relations Humans Inner mitochondrial membrane reference range plasma Chromatography High Pressure Liquid Sex Characteristics Chromatography Plasma samples Chemistry Age Factors Healthy subjects General Medicine Endocrinology healthy subjects Female Acetylcarnitine Blood Chemical Analysis Homeostasis medicine.drug |
| Zdroj: | Annals of Clinical Biochemistry: International Journal of Laboratory Medicine. 45:585-592 |
| ISSN: | 1758-1001 0004-5632 |
| DOI: | 10.1258/acb.2008.008045 |
| Popis: | Backgroundl-carnitine is an endogenous substance, vital in the transport of fatty acids across the inner mitochondrial membrane for oxidation. Disturbances in carnitine homeostasis can have a significant impact on human health; therefore, it is critical to define normal endogenous concentrations for l-carnitine and its esters to facilitate the diagnosis of carnitine deficiency disorders. This study was conducted to determine the normal concentrations of a number of carnitines in healthy adults using three analytical methods. The impact of age and gender on carnitine concentrations was also examined.MethodsBlood samples were collected from 60 healthy subjects of both genders and various ages. Plasma samples were analysed for endogenous carnitine concentrations by radioenzymatic assay, high-performance liquid chromatography and electrospray tandem mass spectrometry.ResultsPrecision and accuracy of results obtained for each assay were within acceptable limits. Average endogenous concentrations obtained from the three analytical methods in this study were in the range of 38–44, 6–7 and 49–50 μmol/L for l-carnitine, acetyl-l-carnitine and total carnitine, respectively. Comparison of results between the genders indicated that males had significantly higher endogenous plasma l-carnitine and total carnitine concentrations than females. Age was found to have no impact on plasma carnitine concentrations.ConclusionThese results are useful in the evaluation of biochemical or metabolic disturbances and in the diagnosis and treatment of patients with carnitine deficiency. |
| Databáze: | OpenAIRE |
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