Gadolinium-Incorporated Carbon Nanodots for T 1 -Weighted Magnetic Resonance Imaging

Autor: Da Zhang, Belén Ballesteros, Hanyu Liang, Giacomo Reina, Juan Li, Shi Guo, Alberto Bianco, Ding-Kun Ji, Cécilia Ménard-Moyon
Přispěvatelé: Centre National de la Recherche Scientifique (France), Ministerio de Economía y Competitividad (España), Generalitat de Catalunya, Immunopathologie et chimie thérapeutique (ICT), Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
ACS Applied Nano Materials
ACS Applied Nano Materials, American Chemical Society, 2021
ISSN: 2574-0970
Popis: The design and development of contrast agents for magnetic resonance imaging (MRI) with improved chemical stability and higher contrasting capability for clinical translation compared to conventional contrast agents are still of great interest. In this study, a facile and universal approach was explored for controllable functionalization of red-emissive carbon nanodots (RCNDs) with diethylenetriaminepentaacetic anhydride (DTPA) for chelation of gadolinium. A series of accurate characterizations were used to control each step of the synthesis. The functionalization did not alter the band gap of the carbon nanodots, preserving their inherent far-red fluorescence. The as-prepared RCND-DTPA-Gd displayed a high colloidal stability with negligible Gd leakage. The nanodots also showed a better magnetic resonance relaxivity than commercial MRI agents. RCND-DTPA-Gd had good biocompatibility in vivo even at high doses. The systemically injected RCND-DTPA-Gd were found to be efficiently excreted through the renal route, a feature that further minimizes the potential toxicity risks. All these properties suggest that carbon nanodots can be well designed as efficient carriers of Gd, resulting in potential clinical tools as dual MRI/fluorescence functional probes for imaging applications. The approach described here could pave the pathway to a flexible strategy for the controllable functionalization of small-sized nanoparticles including carbon dots, rendering them more versatile. This work is expected to promote the future translation of carbon nanodots into clinical trials.
We gratefully acknowledge the Centre National de la Recherche Scientifique (CNRS) and International Center for Frontier Research in Chemistry (icFRC), and financial support from the Agence Nationale de la Recherche (ANR) through the LabEx project Chemistry of Complex Systems (ANR-10-LABX-0026_CSC) within the Investissement d’Avenir program (ANR-10-120 IDEX-0002-02). We wish to thank D. Lamon for injecting the mice and collecting the samples for the biochemical analyses, C. Royer and V. Demais for help with TEM analyses at the “Plateforme Imagerie in vitro” at the Center of Neurochemistry (INCI, Strasbourg, France), D. Ihiawakrim for HRTEM measurements, and S. Guo for his help in the XPS analyses. ICN2 acknowledges the financial support from the Spanish Ministry of Economy and Competitiveness through the “Severo Ochoa” Programme for Centres of Excellence in R&D (SEV-2017-0706). B.B. acknowledges funding from Generalitat de Catalunya 2017 SGR 327. We wish to thank the Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fujian Key Laboratory of Integrative Medicine on Geriatrics, and Prof. Jun Peng for the use of 7 T small-animal MRI scanner.
Databáze: OpenAIRE
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