Expression of heme oxygenase-1, hypoxia inducible factor-1α, and ubiquitin in peripheral inflammatory cells from patients with coronary heart disease
| Autor: | Chunjiang Tan, Yu-Guang Li, Song-Ming Chen, Guo-Hong Zhang, Dong-ming Wang |
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| Rok vydání: | 2009 |
| Předmět: |
Male
medicine.medical_specialty Clinical Biochemistry Angiotensin-Converting Enzyme Inhibitors Coronary Disease Protein degradation chemistry.chemical_compound Ubiquitin Internal medicine Heat shock protein medicine Humans Lymphocytes RNA Messenger Myocardial infarction Heme Inflammation biology Reverse Transcriptase Polymerase Chain Reaction Unstable angina Gene Expression Profiling Biochemistry (medical) General Medicine Middle Aged Hypoxia (medical) Hypoxia-Inducible Factor 1 alpha Subunit medicine.disease Heme oxygenase Endocrinology chemistry Leukocytes Mononuclear biology.protein Female Hydroxymethylglutaryl-CoA Reductase Inhibitors medicine.symptom Angiotensin II Type 1 Receptor Blockers Heme Oxygenase-1 |
| Zdroj: | Clinical Chemistry and Laboratory Medicine. 47 |
| ISSN: | 1437-4331 1434-6621 |
| Popis: | The increased expression of heme oxygenase-1 content, a stress-response protein, directly correlates with the incidence of coronary heart disease. Down-regulation of hypoxia inducible factor-1α activity, a major downstream effector of the signaling pathways activated by hypoxia, increases cell survival after hypoxia. The ubiquitin system, a non-lysosomal pathway of protein degradation, is involved in processes of coronary heart disease. The aim of this study was to investigate the expression of heme oxygenase-1, hypoxia inducible factor-1α, and ubiquitin in both monocytes and lymphocytes isolated from patients at the mRNA and protein levels in different stages of coronary heart disease and their possible correlation.: A total of 90 patients with coronary heart disease (30 acute myocardial infarction, 30 unstable angina pectoris, and 30 stable angina pectoris) were selected, and 30 cases with normal coronary artery served as controls. The mRNA and protein expression of heme oxygenase-1, hypoxia inducible factor-1α, and ubiquitin in monocytes and lymphocytes were examined by semi-quantitative reverse transcriptase polymerase chain reaction and Western blotting, respectively.: The mRNA expression of heme oxygenase-1 and ubiquitin was associated with the severity of coronary heart disease (p: These data suggest that heme oxygenase-1, hypoxia inducible factor-1α, and ubiquitin are involved in the development and progression of coronary heart disease and thus may be useful biomarkers for coronary heart disease.Clin Chem Lab Med 2009;47:327–33. |
| Databáze: | OpenAIRE |
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