A2 (N2) Neuraminidase of the X-7 Influenza Virus Recombinant: Determination of Molecular Size and Subunit Composition of the Active Unit
| Autor: | D. J. Bucher, Edwin D. Kilbourne |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1972 |
| Předmět: |
Genetics
Microbial Sucrose Protein subunit Immunology Orthomyxoviridae Neuraminidase Biology Microbiology Virus chemistry.chemical_compound Viral Proteins Tetramer Virology Neuraminic acid Animal Viruses Centrifugation Density Gradient Sodium dodecyl sulfate Recombination Genetic Chromatography Molecular mass Sodium Dodecyl Sulfate biology.organism_classification Electrophoresis Disc Molecular Weight Dithiothreitol Biochemistry chemistry Spectrophotometry Insect Science biology.protein Peptides |
| Popis: | Neuraminidase activity of influenza virus was directly seen on sodium dodecyl sulfate polyacrylamide gels with the aid of the synthetic substrate, methoxyphenol neuraminic acid. Neuraminidase (NA) appeared as a high-molecular-weight fraction with a size in the range of 220,000 to 250,000 daltons. Isolation of this fraction from the X-7 strain of influenza virus, dissociation with sodium dodecyl sulfate, and reduction showed the presence of two polypeptides of 66,000 (NA 1 ) and 58,000 (NA 2 ) molecular weights in equimolar concentration. We postulate that the minimum active unit for the viral A 2 neuraminidase is a tetramer composed of two NA 1 and two NA 2 subunits. |
| Databáze: | OpenAIRE |
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