Date seeds alleviate behavioural and neuronal complications of metabolic syndrome in rats
| Autor: | Morgane Perrotte, Houssem Dkhili, Saloua El Fazaa, Charles Ramassamy, Michele Veronique El May, Dalila Rahali, Narges El Golli, Asma Korsan, Yosra Dallagi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Physiology
business.industry First line food and beverages Hippocampus 030209 endocrinology & metabolism General Medicine medicine.disease_cause medicine.disease 03 medical and health sciences 0302 clinical medicine 030220 oncology & carcinogenesis Physiology (medical) Medicine sense organs Dietary change Metabolic syndrome business Oxidative stress |
| Zdroj: | Archives of Physiology and Biochemistry. 129:582-596 |
| ISSN: | 1744-4160 1381-3455 |
| Popis: | Unhealthy dietary habits can play a crucial role in metabolic damages, promoting alteration of neural functions through the lifespan. Recently, dietary change has been perceived as the first line intervention in prevention and/or treatment of metabolic damages and related diseases. In this context, our study was designed to assess the eventual therapeutic effect of date seeds administration on memory and learning and on neuronal markers in a rat Metabolic Syndrome model. For this purpose, 32 adult male Wistar rats were fed with standard diet or high-fat high-sugar diet during ten weeks. After this, 16 rats were sacrified and the remaining rats received an oral administration of 300 mg of date seeds/kg of body weight during four supplementary weeks. Before sacrifice, we evaluate cognitive performances by the Barnes maze test. Afterwards, neuronal, astrocytic, microtubular and oxidative markers were investigated by immunoblotting methods. In Metabolic syndrome rats, results showed impairment of spatial memory and histological alterations. We identified neuronal damages in hippocampus, marked by a decrease of NeuN and an increase of GFAP and pTau396. Finally, we recorded an increase in protein oxidation and lipid peroxidation, respectively identified by an up-regulation of protein carbonyls and 4-HNe. Interestingly, date seeds administration improved these behavioural, histological, neuronal and oxidative damages highlighting the neuroprotective effect of this natural compound. Liquid Chromatography-Mass Spectrometry (LC-MS) identified, in date seeds, protocatechuic acid, caffeoylshikimic acid and vanillic acid, that could potentially prevent the progression of neurodegenerative diseases, acting through their antioxidant properties. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|