Expression profile of malignant and non-malignant diseases of the thyroid gland reveals altered expression of a common set of genes in goiter and papillary carcinomas
| Autor: | Alex F. Carvalho, Luiz Paulo Kowalski, Juan P. Díaz, Marcos Brasilino de Carvalho, Roberto Hirata, Fernando Augusto Soares, Beatriz S. Stolf, Maria Paula Curado, Luiz F. L. Reis, Helena Brentani, Márcia Dellamano, Cintia M. Abreu, Waleska Kerllen Martins, Maria Betania Mahler-Araujo, Artur Fabri |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
endocrine system
Cancer Research Pathology medicine.medical_specialty Goiter endocrine system diseases Adenoma Thyroid Gland Biology Complementary DNA medicine Carcinoma Humans Thyroid Neoplasms Gene Oligonucleotide Array Sequence Analysis Tissue microarray Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Thyroid Intracellular Signaling Peptides and Proteins medicine.disease Carcinoma Papillary Gene expression profiling medicine.anatomical_structure Oncology Tissue Array Analysis Carrier Proteins Cyclin-Dependent Kinase Inhibitor p27 |
| Zdroj: | Cancer Letters. 227:59-73 |
| ISSN: | 0304-3835 |
| Popis: | Using cDNA microarrays with 3800 cDNA fragments, we determined the expression profile of normal thyroid tissue, goiter, adenoma and papillary carcinoma (10 samples from each class). After background correction and statistical analysis, we identified a set of 160 genes as being differentially expressed in all pair-wise comparisons. Here we demonstrate that, at least on the basis of these differentially expressed genes, a positive correlation between goiter and papillary carcinomas could be observed. We identified a common set of genes whose expression is diminished in both goiter and papillary carcinomas as compared to normal thyroid tissue. Moreover, no genes with inverse correlation in samples from goiter and papillary carcinomas could be detected. Using Real-Time PCR and/or tissue microarrays, we confirmed the altered expression of some of the identified genes. Of notice, we demonstrate that the reduced mRNA levels of p27(kip1) observed in papillary carcinomas as compared to either goiter or normal thyroid tissues (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect