5-HT2B receptor mRNA in guinea pig superior cervical ganglion
| Autor: | C J Watkins, Nigel R. Newberry, T P Flanigan, A Volenec |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
medicine.medical_specialty Superior cervical ganglion Sequence analysis Guinea Pigs Molecular Sequence Data Superior Cervical Ganglion Biology Polymerase Chain Reaction Rats Sprague-Dawley Guinea pig Internal medicine Gene expression medicine Animals Humans RNA Messenger Receptor Brain Chemistry Messenger RNA Base Sequence General Neuroscience 5-HT2 receptor Molecular biology 5-HT2B receptor Rats Endocrinology Receptors Serotonin Oligonucleotide Probes |
| Zdroj: | NeuroReport. 7:2909-2912 |
| ISSN: | 0959-4965 |
| DOI: | 10.1097/00001756-199611250-00020 |
| Popis: | Primers for 5-HT2A, 5-HT2B and 5-HT2C receptor mRNAs were used in reverse transcriptase-linked polymerase chain reactions (RT-PCR) to determine the presence of these transcripts in the guinea pig superior cervical ganglion. This was done to help identify an as yet unknown 5-HT2-like receptor which, in addition to 5-HT2A receptors, mediates a slow depolarization of this preparation. PCR products corresponding to 5-HT2A and 5-HT2B, but not 5-HT2C, receptor mRNA could readily be detected. Subsequent sequence analysis of these products confirmed that the 5-HT2A band corresponded to part of the guinea pig 5-HT2A receptor and the 5-HT2B band probably represents a portion of the guinea pig 5-HT2B receptor. The latter sequence shares greater homology with an equivalent region of the human than the rat 5-HT2B receptor. |
| Databáze: | OpenAIRE |
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