CK2 functionally interacts with AKT/PKB to promote the β-catenin-dependent expression of survivin and enhance cell survival

Autor: Daniela P. Ponce, Ignacio Niechi, Ricardo Armisen, Marcelo Antonelli, Mario Galindo, Jose L. Maturana, Eduardo Silva, Julio C. Tapia, Katherine Marcelain, Pablo Cabello, Roger Yefi
Rok vydání: 2011
Předmět:
Zdroj: Molecular and Cellular Biochemistry. 356:127-132
ISSN: 1573-4919
0300-8177
DOI: 10.1007/s11010-011-0965-4
Popis: β-Catenin is crucial in the canonical Wnt signaling pathway. This pathway is up-regulated by CK2 which is associated with an enhanced expression of the antiapoptotic protein survivin, although the underlying molecular mechanism is unknown. AKT/PKB kinase phosphorylates and promotes β-catenin transcriptional activity, whereas CK2 hyperactivates AKT by phosphorylation at Ser129; however, the role of this phosphorylation on β-catenin transcriptional activity and cell survival is unclear. We studied in HEK-293T cells, the effect of CK2-dependent hyperactivation of AKT on cell viability, as well as analyzed β-catenin subcellular localization and transcriptional activity and survivin expression. CK2α overexpression led to an augmented β-catenin-dependent transcription and protein levels of survivin, and consequently an enhanced resistance to apoptosis. However, CK2α-enhancing effects were reversed when an AKT mutant deficient in Ser129 phosphorylation by CK2 was co-expressed. Therefore, our results strongly suggest that CK2α-specific enhancement of β-catenin transcriptional activity as well as cell survival may depend on AKT hyperactivation by CK2.
Databáze: OpenAIRE
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