Field and laboratory assessments of sugarcane mutants selected in vitro for resistance to imazapyr herbicide
| Autor: | S. J. Snyman, A. C. Koch, R. S. Rutherford, M.P. Watt, K.Z. Maphalala |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Sucrose Ethyl methanesulfonate lcsh:Biotechnology lcsh:Plant culture Biology 01 natural sciences chemistry.chemical_compound lcsh:TP248.13-248.65 lcsh:SB1-1110 General Environmental Science Acetolactate synthase Mutation breeding Acetoin Lethal dose mutation breeding 04 agricultural and veterinary sciences Imazapyr Horticulture chemistry Agronomy Germination 040103 agronomy & agriculture biology.protein imidazolinone 0401 agriculture forestry and fisheries General Earth and Planetary Sciences ethyl methanesulfonate 010606 plant biology & botany |
| Zdroj: | Crop Breeding and Applied Biotechnology, Volume: 17, Issue: 2, Pages: 107-114, Published: JUN 2017 Crop Breeding and Applied Biotechnology v.17 n.2 2017 Crop Breeding and Applied Biotechnology Sociedade Brasileira de Melhoramento de Plantas instacron:CBAB Crop Breeding and Applied Biotechnology, Vol 17, Iss 2, Pp 107-114 (2017) |
| Popis: | Seven imazapyr-tolerant mutant sugarcane plants, previously gener -ated by in vitro mutagenesis, were studied. The imazapyr concentrations re-quired to inhibit their acetolactate synthase (ALS basal activity) (IC 50 as μmoles acetoin h -1 mg -1 protein) were 0.77 – 5.36 times greater than that of the N12 ‘parent’. The basal ALS activities of Mut1 and Mut6 were 1.4-fold higher than that of N12. When the mutants were sprayed with Arsenal ® GEN 2 (312 and 624 g a.i. imazapyr ha -1 ), 2 months after field planting, and evaluated 9 months later, live stalk height and number were significantly lowest in Mut2, Mut3 and the control N12. No differences in sucrose, fibre and estimated yield were observed amongst lines in untreated plots. Mutant plants germinated and grew in soil treated with the herbicide (at the lethal dose of 1248 g a.i. ha -1 ). The Mut lines tested in this study offer improved options for weed control. Key words: Acetolactate synthase, ethyl methanesulfonate, imidazolinone |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|