| Autor: |
Yu Wang, Tao Jiang, Zhaoqian Teng, Hao Wu, Chen Zhao, Chenze Zhang, Fujia Wu |
| Rok vydání: |
2023 |
| DOI: |
10.1158/0008-5472.22423338.v1 |
| Popis: |
Supplementary Figure S1. Generation and characterization of cell lines for high content screening and GLI-luciferase reporter assays. Supplementary Figure S2. A high content screening identifies compound candidates that inhibit GLI2 ciliary localization. Supplementary Figure S3. Additional prostaglandins were examined in the GLI-luciferase reporter assay using 3T3/GLI-luc/GLI2 cells. Supplementary Figure S4. Comparative examination of dose-dependent effects of the prostaglandins identified from high content screening on the GLI-luciferase reporter activity mediated by SMO-WT, SMO-D473H and SMO-W535L respectively. Supplementary Figure S5. Examination of prostaglandins against SHH-N/SAG-induced HH pathway activity. Supplementary Figure S6. A CRE-luciferase reporter assay was used to examine cAMP-PKA levels. Supplementary Figure S7. Generation and examination of EP4 knockout monoclonal cell lines. Supplementary Figure S8. Examination of the expression of EP receptors and the activity of PGE1 in DAOY cells and growth comparison of wild-type DAOY cells and GLI2-overexpressed DAOY cells. Supplementary Figure S9. Molecular analyses of Med-113FH and Med-314FH xenografts. Supplementary Figure S10. Examination of the activities of PGE1 metabolites. Supplementary Figure S11. Examination of additional clinical prostaglandins. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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