Association of Alterations in NF-κB Moieties with HIV Type 1 Proviral Latency in Certain Monocytic Cells
Autor: | Lingxun Duan, Omar Bagasra, Joseph W. Oakes, Roger J. Pomerantz |
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Rok vydání: | 1994 |
Předmět: |
viruses
Molecular Sequence Data Immunology Human immunodeficiency virus (HIV) Biology medicine.disease_cause Monocytes Virus Cell Line chemistry.chemical_compound Proviruses Virology medicine Humans Latency (engineering) Transcription factor Cellular proteins Binding Sites Base Sequence Monocyte NF-kappa B Transcription Factor RelA NF-kappa B p50 Subunit virus diseases NF-κB Virus Latency Infectious Diseases medicine.anatomical_structure chemistry Cell culture HIV-1 Electrophoresis Polyacrylamide Gel Oligonucleotide Probes |
Zdroj: | AIDS Research and Human Retroviruses. 10:1213-1219 |
ISSN: | 1931-8405 0889-2229 |
Popis: | Human immunodeficiency virus type 1 (HIV-1) replication is controlled by a complex array of virally encoded and cellular proteins. A wide spectrum of levels of HIV-1 expression have been demonstrated in various cells, both in cell culture and in vivo. Molecular mechanisms leading to restricted HIV-1 replication may differ between certain cell types. It is now demonstrated that HIV-1 proviral latency in the monocytic cell line U1, in which only extremely low levels of HIV-1 expression are detected in the baseline unstimulated state, is associated with alterations in nuclear factor-kappa B (NF-kappa B) moieties demonstrated in these cells by electrophoretic mobility shift assays (EMSAs) and in situ UV cross-linking studies. A predominance of p50 NF-kappa B moieties and possibly p50 homodimers or closely related species, rather than the p50-p56 heterodimer of NF-kappa B that is the predominant NF-kappa B species in most T lymphocytic and monocytic cells, is demonstrated in the nuclei of U1 cells. This pattern of NF-kappa B-related moieties differs from the latently infected T lymphocytic cell line ACH-2, and from the U937 monocytic line, the parental cell line of the U1 cellular clone. As such, these data suggest that different proximal mechanisms may lead to restricted HIV-1 replication in various cell types. |
Databáze: | OpenAIRE |
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