Evaluation of the influence of S-adenosylmethionine on systemic and hepatic effects of prednisolone in dogs
| Autor: | Sharon A. Center, Karen L. Warner, Jennifer McCabe, Polly Foureman, Walter E. Hoffmann, Hollis N. Erb |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
S-Adenosylmethionine medicine.medical_specialty Prednisolone Administration Oral Biological Availability Pilot Projects Total alkaline phosphatase Placebo Thiobarbituric Acid Reactive Substances Dogs Double-Blind Method Internal medicine medicine TBARS Animals Prospective Studies Prospective cohort study Glucocorticoids Cross-Over Studies General Veterinary business.industry Erythrocyte Membrane General Medicine Alkaline Phosphatase Glutathione Crossover study Globins Reverse order Endocrinology Liver Female business Glucocorticoid medicine.drug |
| Zdroj: | American Journal of Veterinary Research. 66:330-341 |
| ISSN: | 0002-9645 |
| DOI: | 10.2460/ajvr.2005.66.330 |
| Popis: | Objective—To evaluate the influence of a 1,4- butanedisulfonate stable salt of S-adenosylmethionine (SAMe) administered orally on clinicopathologic and hepatic effects induced by long-term administration of prednisolone in dogs. Animals—12 healthy dogs. Procedure—Following a pilot study (4 dogs), 2 groups of 4 dogs received prednisolone (2.2 mg/kg) orally once daily (84-day trial). One group received SAMe (20 mg/kg/d divided in 2 doses) for 42 days and then a placebo for 42 days; the other group received treatments in the reverse order. Before and during the trial, numerous variables were monitored, including serum total alkaline phosphatase (ALP) and glucocorticoid- induced ALP (G-ALP) activities, serum haptoglobin concentration, and total and oxidized glutathione (TGSH and GSSG) and thiobarbiturate-reacting substances (TBARS) concentrations in erythrocytes and liver tissue (days 0, 42, and 84). Hepatic specimens also were examined microscopically. Results—The stable salt of SAMe was biologically available; plasma concentrations of SAMe or prednisolone were not affected by coadministration. Compared with baseline values, serum ALP and GALP activities and haptoglobin concentrations increased and erythrocyte GSSG and TBARS concentrations decreased with both treatments. Erythrocyte TGSH concentration decreased with the prednisolone- placebo treatment. Administration of SAMe appeared to conserve erythrocyte TGSH values and did not inhibit hepatocyte glycogen vacuolation but increased hepatic TGSH concentration and improved the hepatic tissue GSSG:TGSH ratio. Conclusions and Clinical Relevance—In dogs, administration of 20 mg of SAMe/kg/d may mitigate the apparent pro-oxidant influences of prednisolone but did not block development of classic clinicopathologic or histologic features of vacuolar hepatopathy. (Am J Vet Res 2005;66:330–341) |
| Databáze: | OpenAIRE |
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