Optical Biosensor Platforms Display Varying Sensitivity for the Direct Detection of Influenza RNA
| Autor: | Jason D. Gans, James Theiler, Jessica Z. Kubicek-Sutherland, Brian T. Foley, Zachary R. Stromberg, Daniel Jacobsen, Loreen R. Stromberg, Kiersten D. Lenz, Karina Yusim, Samantha J. Courtney, Adán Myers y Gutiérrez |
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| Rok vydání: | 2021 |
| Předmět: |
flow cytometer
Computer science In silico Clinical Biochemistry detection Computational biology Biosensing Techniques waveguide biosensor Sensitivity and Specificity Virus Article Molecular beacon Pandemic Influenza Human diagnostics Humans RNA General Medicine Orthomyxoviridae GenBank Nucleic acid influenza Biosensor Nucleic Acid Amplification Techniques TP248.13-248.65 Biotechnology |
| Zdroj: | Biosensors Biosensors, Vol 11, Iss 367, p 367 (2021) Volume 11 Issue 10 |
| ISSN: | 2079-6374 |
| Popis: | Detection methods that do not require nucleic acid amplification are advantageous for viral diagnostics due to their rapid results. These platforms could provide information for both accurate diagnoses and pandemic surveillance. Influenza virus is prone to pandemic-inducing genetic mutations, so there is a need to apply these detection platforms to influenza diagnostics. Here, we analyzed the Fast Evaluation of Viral Emerging Risks (FEVER) pipeline on ultrasensitive detection platforms, including a waveguide-based optical biosensor and a flow cytometry bead-based assay. The pipeline was also evaluated in silico for sequence coverage in comparison to the U.S. Centers for Disease Control and Prevention’s (CDC) influenza A and B diagnostic assays. The influenza FEVER probe design had a higher tolerance for mismatched bases than the CDC’s probes, and the FEVER probes altogether had a higher detection rate for influenza isolate sequences from GenBank. When formatted for use as molecular beacons, the FEVER probes detected influenza RNA as low as 50 nM on the waveguide-based optical biosensor and 1 nM on the flow cytometer. In addition to molecular beacons, which have an inherently high background signal we also developed an exonuclease selection method that could detect 500 pM of RNA. The combination of high-coverage probes developed using the FEVER pipeline coupled with ultrasensitive optical biosensors is a promising approach for future influenza diagnostic and biosurveillance applications. |
| Databáze: | OpenAIRE |
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