Characterization of sexual progenies of male-sterile somatic cybrids between Brassica napus and Brassica tournefortii

Autor: J.-H. Liu Clarke, Kristina Glimelius, M. Landgren, A.-M. Chèvre
Přispěvatelé: ProdInra, Migration, UMR 0118 UMR INRA / ENSAR : Génétique et amélioration des plantes, Institut National de la Recherche Agronomique (INRA)-Génétique et amélioration des plantes (G.A.P.)-UMR INRA / ENSAR : Génétique et amélioration des plantes (RENN UMR GENET AMELIOR PLANTES)
Rok vydání: 1999
Předmět:
Zdroj: TAG Theoretical and Applied Genetics
TAG Theoretical and Applied Genetics, Springer Verlag, 1999, 99, pp.605-610
ISSN: 1432-2242
0040-5752
DOI: 10.1007/s001220051275
Popis: Cytogenetic studies were performed on four male-sterile progenies derived from four different cybrids produced between Brassica napus and B. tournefortii using the donor-recipient protoplast fusion method. The objective of these studies was to characterize the nuclear constitution of the plants. Mitotic investigation revealed that three of the four male-sterile lines had 38 chromosomes, which is equal to that of B. napus. The fourth line, C6, had variable chromosome numbers, ranging from 39 to 42 in different plants. The meiotic behavior in each progeny varied distinctly. Of the plants having 38 chromosomes, fairly high chromosome pairing, on average 18.08 bivalents per cell, was detected at metaphase-I. However, univalents with an average of 1.39 per cell, and very low frequencies of trivalents and/or tetravalents, were also observed in the lines. These results revealed that male-sterile cybrid lines were obtained with 38 chromosomes and a relatively high level of chromosome-pairing ability, indicating their potential for establishing a stable male-sterile rapeseed line.
Databáze: OpenAIRE
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