Production of Interleukin-12 as a Self-Processing 2A Polypeptide
| Autor: | Paul J. Chaplin, Martin D. Ryan, Evelyn B. Camon, Barnardo Villarreal-Ramos, R.A. Collins, Micheal Flint |
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| Rok vydání: | 1999 |
| Předmět: |
2a peptide
Multiprotein complex medicine.medical_treatment Genetic Vectors Immunology Cell Biology Biology Cleavage (embryo) Interleukin-12 Virus Viral Proteins Cytokine Biochemistry Virology COS Cells medicine Interleukin 12 Animals Humans Cattle Protein Processing Post-Translational Gene Cells Cultured Self processing |
| Zdroj: | Journal of Interferon & Cytokine Research. 19:235-241 |
| ISSN: | 1557-7465 1079-9907 |
| DOI: | 10.1089/107999099314162 |
| Popis: | Interleukin-12 (IL-12) is a heterodimeric cytokine composed of two disulfide-linked subunits (p40 and p35) encoded by separate genes. We used the apparent autocleavage property of a 2A peptide from the foot-and-mouth disease virus (FMDV) to express bovine (Bo) IL-12 as a self-processing polypeptide (p402Ap35). We demonstrate that 2A will mediate the cleavage of p402Ap35 into two separate subunits in a manner similar to that observed during the processing of the FMDV polypeptide. Furthermore, this 2A polypeptide encoded a functional heterodimer, which elicited activities associated with IL-12 in other species. We propose that this strategy of self-processing polypeptides may be used in many applications where the coordinated and stoichiometric expression of complex proteins is required. |
| Databáze: | OpenAIRE |
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