The effects of glucose concentrations associated with lipopolysaccharide and interferon-gamma stimulus on mediators' production of RAW 264.7 cells
| Autor: | Ana Paula C. Cantuária, Mirna S. Freire, Stella Maris de Freitas Lima, Taia Maria Berto Rezende, Jeeser Alves de Almeida, Octavio L. Franco, Tarsila de Moura Figueiredo |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides Chemokine medicine.medical_specialty Time Factors Lipopolysaccharide Cell Survival Immunology 030209 endocrinology & metabolism Nitric Oxide Biochemistry 03 medical and health sciences chemistry.chemical_compound Interferon-gamma Mice 0302 clinical medicine Immune system Internal medicine medicine Immunology and Allergy Animals Interferon gamma Viability assay Molecular Biology RAW 264.7 Cells Chemokine CCL2 biology Dose-Response Relationship Drug Macrophages Interleukin Hematology 030104 developmental biology Endocrinology Glucose chemistry biology.protein Cytokines Tumor necrosis factor alpha Inflammation Mediators medicine.drug |
| Zdroj: | Cytokine. 107 |
| ISSN: | 1096-0023 |
| Popis: | Diabetes mellitus (DM) is a metabolic disorder that results in the impairment of the metabolism of carbohydrates, proteins and lipids. It can give rise to various complications, mainly caused by chronic exposure of cells to high glucose concentrations, including changes in the immune response processes. The aim of this study was to verify the chemokine and cytokines production profile in the presence of different glucose concentrations and infection/inflammatory stimuli. To this end, cell viability and the production of chemokines, cytokines and nitric oxide (NO) were analyzed in RAW 264.7 cell culture. Results demonstrated that there was no change in cell viability after 6, 24 and 72 h. Different stimuli were unable to modify the monocyte chemoattractant protein (MCP)-1 and tumor necrosis factor (TNF)-α production. Groups stimulated with lipopolysaccharides (LPS) and LPS and recombinant interferon (rIFN)-γ down-regulated interleukin (IL)-1α, IL-10 and IL-12 and up-regulated IL-6 production. NO production maintained a pattern of increase, according to the increase in glucose concentrations, reaching its peak at 72 h. In summary, the results demonstrated that high glucose concentrations alone may be sufficient to alter the in vitro mediators’ production of RAW 264.7 cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect