Islet Cell Hyperplasia in an Aged Spider Monkey (Ateles paniscus)
| Autor: | A. J. Herron, S. R. Brunnert, Norman H. Altman |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
0301 basic medicine
Aging Pathology medicine.medical_specialty 040301 veterinary sciences Biology Glucagon Immunoenzyme Techniques 0403 veterinary science Islets of Langerhans 03 medical and health sciences Internal medicine medicine Animals Pancreatic polypeptide geography Hyperplasia geography.geographical_feature_category General Veterinary Immunoperoxidase Pancreatic islets Monkey Diseases 04 agricultural and veterinary sciences Islet medicine.disease Immunohistochemistry Staining Microscopy Electron 030104 developmental biology medicine.anatomical_structure Endocrinology Cebidae Female Pancreas |
| Zdroj: | Veterinary Pathology. 27:372-374 |
| ISSN: | 1544-2217 0300-9858 |
| DOI: | 10.1177/030098589002700513 |
| Popis: | Proliferation of pancreatic endocrine tissue is a poorly defined phenomenon in animals. Hyperplasia of the pancreatic islets has been reported in the Golden hamster2 and a variety of mouse strains, including the yellow (C3H x VY-AVY) hybrid F, female mouse.’ In human beings and in rats, hyperplastic islets are associated with pancreatic fibro~is.’.~ In the rat, advancing age7 and repeated breeding are also related factors. This is partially due to the increased insulin demand in the face of declining islet cell function.8 Two cases of “islet cell adenomatosis” have been reported in the chimpanzee6 with minimal clinical or morphologic descriptive detail. Little information is available on the pathology of the pancreas in New World primates6 This report describes how we employed immunocytochemistry and electron microscopy to characterize a case of marked islet cell hyperplasia. A greater than 20-year-old, female, wild-caught spider monkey (Ateles panzscus) that had been kept in captivity for 15 years in a private collection died suddenly. At necropsy, the monkey was severely emaciated, with marked skeletal muscle wasting of the hind limbs. Other necropsy findings included an enlarged, bronzed liver, a markedly thickened and hemorrhagic colon and cecum, and a multinodular pancreas. The pancreas was normal in size with numerous 2-3 mm nodules. All organs were fixed in 10% neutral buffered formalin, routinely processed, and stained with hematoxylin and eosin. Sections of pancreas were stained with Masson’s trichrome and Gomori’s aldehyde fuchsin. Additional 5 pm sections of the pancreas were stained by avidin-biotin-complex immunoperoxidase methods for glucagon, insulin, pancreatic polypeptide, somatostatin, gastrin, and serotonin. Formalin-fixed tissue was prepared for electron microscopy by post-fixation in 1% osmium tetroxide, dehydrated with graded alcohols, and embedded in epoxy resin. Briefly, in the immunoperoxidase method, the slides were heated to melt the paraffin, dewaxed in xylene, and hydrated through graded ethanols. Endogenous peroxidase activity was blocked by treating the sections with hydrogen peroxide in methanol for 20 minutes. The slides were rinsed with tap water and phosphate buffered saline, then treated with normal horse serum for 20 minutes to reduce non-specific background staining. After rinsing, the sections were incubated for 30 minutes with primary antisera dilutions directed at either glucagon, insulin, pancreatic polypeptide, somatostat |
| Databáze: | OpenAIRE |
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