Simultaneous saccharification and co-fermentation of dry diluted acid pretreated corn stover at high dry matter loading: Overcoming the inhibitors by non-tolerant yeast
| Autor: | Yao-Dong Huang, Jie Bao, Lei Qin, Ying-Jin Yuan, Jia-Qing Zhu, Jian Zhang, Bing-Zhi Li, Wen-Chao Li |
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| Rok vydání: | 2015 |
| Předmět: |
Co-fermentation
Environmental Engineering Time Factors Bioconversion Bioengineering Saccharomyces cerevisiae Xylose Lignin Zea mays chemistry.chemical_compound Bioreactors Dry matter Ethanol fuel Food science Biomass Desiccation Waste Management and Disposal Stover Ethanol Renewable Energy Sustainability and the Environment food and beverages General Medicine Corn stover chemistry Biochemistry Fermentation Solvents Acids |
| Zdroj: | Bioresource technology. 198 |
| ISSN: | 1873-2976 |
| Popis: | Dry dilute acid pretreatment (DDAP) is a promising method for lignocellulose bioconversion, although inhibitors generated during the pretreatment impede the fermentation severely. We developed the simultaneous saccharification and co-fermentation (SScF) of DDAP pretreated biomass at high solid loading using xylose fermenting Saccharomyces cerevisiae, SyBE005. Effect of temperature on SScF showed that ethanol yield at 34°C was 10.2% higher than that at 38°C. Ethanol concentration reached 29.5 g/L at 15% (w/w) dry matter loading, while SScF almost ceased at the beginning at 25% (w/w) dry matter loading of DDAP pretreated corn stover. According to the effect of the diluted hydrolysate on the fermentation of strain SyBE005, a fed-batch mode was developed for the SScF of DDAP pretreated corn stover with 25% dry matter loading without detoxification, and 40.0 g/L ethanol was achieved. In addition, high yeast inoculation improved xylose utilization and the final ethanol concentration reached 47.2 g/L. |
| Databáze: | OpenAIRE |
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