The effect of light exposure on the cleavage rate and implantation capacity of preimplantation murine embryos
| Autor: | Janos Schmidt, Timea Judit Csabai, Timea Balassa, Éva Pállinger, Julia Szekeres-Bartho, Zoltan Bognar, József Bódis, Eva Gorgey, Nelli Farkas, Gergely Berta |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male animal structures Light Immunology Spleen Fertilization in Vitro Flow cytometry Andrology 03 medical and health sciences Mice 0302 clinical medicine Pregnancy medicine Immunology and Allergy Animals Embryo Implantation 030219 obstetrics & reproductive medicine TUNEL assay medicine.diagnostic_test Light sensitivity Chemistry Obstetrics and Gynecology Embryo Embryo Mammalian In vitro 030104 developmental biology medicine.anatomical_structure Blastocyst Reproductive Medicine Apoptosis embryonic structures Models Animal DNA fragmentation Female |
| Zdroj: | Journal of reproductive immunology. 132 |
| ISSN: | 1872-7603 |
| Popis: | During assisted reproduction the embryos are subjected to light. We investigated the relationship between light exposure and the developmental- and implantation capacity of mouse embryos. In vitro cultured embryos were exposed to white or red filtered light, then transferred to the uteri of pseudo-pregnant females. The mice were sacrificed on day 8.5 and implantation sites were counted. The number of nucleic acid containing (PI+) extracellular vesicles (EVs) in culture media of light-exposed and control embryos, as well as, the effect of the EVs on IL-10 production of CD8+ spleen cells was determined by flow cytometry. DNA fragmentation in control and light exposed embryos was detected in a TUNEL assay. The effect of light on the expression of apoptosis-related molecules was assessed in an apoptosis array. Light exposure significantly reduced the implantation capacity of the embryos. The harmful effect was related to the wavelength, rather than to the brightness of the light. Culture media of light exposed groups contained significantly higher number of PI + EVs than those of the control embryos, and failed to induce IL-10 production of spleen cells. The number of nuclei with fragmented DNA, was significantly higher in embryos treated with white light, than in the other two groups. In conclusion exposure to white light impairs the implantation potential of in vitro cultured mouse embryos. These effects are partly corrected by using a red filter. Since there is no information on the light sensitivity of human embryos, embryo manipulation during IVF and ICSI should be performed with caution. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect