Detection and Differentiation of Bacterial and Fungal Infection of Neutrophils from Peripheral Blood Using Raman Spectroscopy
| Autor: | Marcel Dahms, Jürgen Popp, Iwan W. Schie, Angelina Pittner, Jan Rüger, Abdullah Saif Mondol, Michael Bauer, Natalie Arend, Ute Neugebauer, Anuradha Ramoji, Oliver Kurzai |
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| Rok vydání: | 2020 |
| Předmět: |
Staphylococcus aureus
Innate immune system Neutrophils Chemistry 010401 analytical chemistry biochemical phenomena metabolism and nutrition Spectrum Analysis Raman 010402 general chemistry 01 natural sciences Peripheral blood 0104 chemical sciences Analytical Chemistry Microbiology symbols.namesake Candida albicans Escherichia coli symbols Animals Humans Raman spectroscopy |
| Zdroj: | Analytical Chemistry. 92:10560-10568 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | Neutrophils are important cells of the innate immune system and the major leukocyte subpopulation in blood. They are responsible for recognizing and neutralizing invading pathogens, such as bacteria or fungi. For this, neutrophils are well equipped with pathogen recognizing receptors, cytokines, effector molecules, and granules filled with reactive oxygen species (ROS)-producing enzymes. Depending on the pathogen type, different reactions are triggered, which result in specific activation states of the neutrophils. Here, we aim to establish a label-free method to indirectly detect infections and to identify the cause of infection by spectroscopic characterization of the neutrophils. For this, isolated neutrophils from human peripheral blood were stimulated in an |
| Databáze: | OpenAIRE |
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