Identifying neuronal correlates of dying and resuscitation in a model of reversible brain anoxia
| Autor: | Vincent Navarro, Antoine Carton-Leclercq, Stéphane Charpier, Séverine Mahon, Shana Diallo, Adrien E. Schramm, Mario Chavez |
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| Přispěvatelé: | Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut du Cerveau = Paris Brain Institute (ICM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), CCSD, Accord Elsevier |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Dying Resuscitation Near-death experience [SDV]Life Sciences [q-bio] Action Potentials Neocortex Biology Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine medicine Repolarization Animals Hypoxia Brain Neuronal excitability ComputingMilieux_MISCELLANEOUS Membrane potential General Neuroscience Pyramidal Cells Brain Depolarization Electroencephalography Cortical neurons Brain anoxia Brain Anoxia [SDV] Life Sciences [q-bio] 030104 developmental biology medicine.anatomical_structure Neuroscience 030217 neurology & neurosurgery Intracellular |
| Zdroj: | Progress in Neurobiology Progress in Neurobiology, Elsevier, 2020, 185, pp.101733. ⟨10.1016/j.pneurobio.2019.101733⟩ Progress in Neurobiology, 2020, 185, pp.101733. ⟨10.1016/j.pneurobio.2019.101733⟩ |
| ISSN: | 0301-0082 |
| DOI: | 10.1016/j.pneurobio.2019.101733⟩ |
| Popis: | International audience; We developed a new rodent model of reversible brain anoxia and performed continuous electrocorticographic (ECoG) and intracellular recordings of neocortical neurons to identify in real-time the cellular and network dynamics that successively emerge throughout the dying-to-recovery process. Along with a global decrease in ECoG amplitude, deprivation of oxygen supply resulted in an early surge of beta-gamma activities, accompanied by rhythmic membrane depolarizations and regular firing in pyramidal neurons. ECoG and intracellular signals were then dominated by low-frequency activities which progressively declined towards isoelectric levels. Cortical neurons during the isoelectric state underwent a massive membrane potential depolarizing shift, captured in the ECoG as a large amplitude triphasic wave known as the "wave-of-death" (WoD). This neuronal anoxic depolarization, associated with a block of action potentials and a loss of cell integrative properties, could however be reversed if brain re-oxygenation was rapidly restored (within 2-3.5 min). The subsequent slow repolarization of neocortical neurons resulted in a second identifiable ECoG wave we termed "wave-of-resuscitation" since it inaugurated the progressive regaining of pre-anoxic synaptic and firing activities. These results demonstrate that the WoD is not a biomarker of an irremediable death and unveil the cellular correlates of a novel ECoG wave that may be predictive of a successful recovery. The identification of real-time biomarkers of onset and termination of cell anoxic insult could benefit research on interventional strategies to optimize resuscitation procedures. |
| Databáze: | OpenAIRE |
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