A Technical Triade for Proteomic Identification and Characterization of Cancer Biomarkers
| Autor: | Andreas Wiesner, Ferdinand von Eggeling, Sven Koscielny, Christian Melle, Ursula Möller, Günther Ernst, Dirk Osterloh, Bettina Schimmel, Annett Bleul, Ralf Bogumil, Karl-Jürgen Halbhuber |
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| Rok vydání: | 2004 |
| Předmět: |
Proteomics
Cancer Research Protein Array Analysis Biology Epithelium S100A9 Metastasis S100A8 Biomarkers Tumor medicine Calgranulin B Humans Calgranulin A Microdissection Gel electrophoresis S100 Proteins Proteolytic enzymes medicine.disease Immunohistochemistry Molecular biology Oncology Head and Neck Neoplasms Carcinoma Squamous Cell Pharynx Cancer biomarkers Cryoultramicrotomy |
| Zdroj: | Cancer Research. 64:4099-4104 |
| ISSN: | 1538-7445 0008-5472 |
| Popis: | Biomarkers are needed to elucidate the biological background and to improve the detection of cancer. Therefore, we have analyzed laser-microdissected cryostat sections from head and neck tumors and adjacent mucosa on ProteinChip arrays. Two differentially expressed proteins (P = 3.34 × 10−5 and 4.6 × 10−5) were isolated by two-dimensional gel electrophoresis and identified as S100A8 (calgranulin A) and S100A9 (calgranulin B) by in-gel proteolytic digestion, peptide mapping, tandem mass spectrometry analysis, and immunodepletion assay. The relevance of these single marker proteins was evaluated by immunohistochemistry. Positive tissue areas were reanalyzed on ProteinChip arrays to confirm the identity of these proteins. As a control, a peak with low P was identified as calgizzarin (S100A11) and characterized in the same way. This technical triade of tissue microdissection, ProteinChip technology, and immunohistochemistry opens up the possibility to find, identify, and characterize tumor relevant biomarkers, which will allow the movement toward the clonal heterogeneity of malignant tumors. Taking this approach, proteins were identified that might be responsible for invasion and metastasis. |
| Databáze: | OpenAIRE |
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