Celecoxib Inhibits Helicobacter pylori-induced Invasion of Gastric Cancer Cells Through an Adenine Nucleotide Translocator-Dependent Mechanism
| Autor: | Shunmei Wan, Dianchun Fang, Chun-Hui Lan, Jun Wang, Lilin Fan, Liuqin Yang, Yafei Zhang, Guo Jun Guo, Rongquan Wang, Shi-Ming Yang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
Helicobacter Infections Metastasis chemistry.chemical_compound Cell Movement Stomach Neoplasms Adenine nucleotide medicine Humans Protein Isoforms Neoplasm Invasiveness Cells Cultured Cell Proliferation Pharmacology Sulfonamides Cyclooxygenase 2 Inhibitors Helicobacter pylori biology Chemistry Adenine nucleotide translocator Anoikis biology.organism_classification medicine.disease Celecoxib Apoptosis Immunology Cancer cell biology.protein Cancer research Pyrazoles Molecular Medicine Growth inhibition Mitochondrial ADP ATP Translocases medicine.drug |
| Zdroj: | Anti-Cancer Agents in Medicinal Chemistry. 13:1267-1272 |
| ISSN: | 1871-5206 |
| DOI: | 10.2174/18715206113139990324 |
| Popis: | Cyclooxygenase-2 (COX-2) inhibitor, celecoxib, causes growth inhibition of human gastric carcinoma cells, but it remains unclear whether celecoxib inhibits Helicobacter pylori-induced invasion of gastric cancer cells. The adenine nucleotide translocator (ANT) is a mitochondrial bi-functional protein. We speculate that ANT-dependent pathways might contribute to H. pylori-induced invasion and metastasis of gastric cancer cells. Therefore, in the present study, we evaluate the effect of celecoxib on H. pylori-induced gastric cancer cell motility and invasion. We also explore the role of ANTs in H. pylori-induced gastric cancer cell motility and invasion of gastric cancer cell line AGS. Our results demonstrate that celecoxib induces anoikis-like apoptosis and suppresses the proliferation and invasion of gastric cancer cells induced by H. pylori in culture. RT-PCR and Western blot analysis indicates that celecoxib upregulates the expression of ANT1 and ANT3; however, celecoxib did not increase the expression of ANT2. Our results suggest that celecoxib could be an effective means for suppressing proliferation and invasion of gastric cancer cells induced by H. pylori through an adenine nucleotide translocator-dependent mechanism. |
| Databáze: | OpenAIRE |
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