Abdominal surgery prior to chronic psychosocial stress promotes spleen cell (re)activity and glucocorticoid resistance
| Autor: | Dominik Langgartner, Sandra Foertsch, Stefan O. Reber |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Lipopolysaccharide lcsh:Medicine Spleen Article 03 medical and health sciences Basal (phylogenetics) chemistry.chemical_compound 0302 clinical medicine Corticosterone Internal medicine Abdomen medicine Splenocyte Animals Viability assay lcsh:Science Glucocorticoids Multidisciplinary business.industry lcsh:R Stressor Chronic inflammation Housing Animal Mice Inbred C57BL 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry Risk factors Chronic Disease Splenomegaly lcsh:Q business 030217 neurology & neurosurgery Stress Psychological Abdominal surgery |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-8 (2020) |
| ISSN: | 2045-2322 |
| Popis: | There is convincing evidence from different mouse models that chronic psychosocial stress promotes splenomegaly, basal and lipopolysaccharide (LPS)-induced in vitro splenocyte activation and insensitivity towards glucocorticoids (GC) in in vitro LPS-treated splenocytes. However, we just recently showed, employing the chronic subordinate colony housing (CSC) paradigm, that bite wounds received during stressor exposure drive these stress-induced spleen changes. As skin wounds induced by planned surgery or physical trauma are more adequately reflecting what chronically stressed humans are likely to experience, it was the objective of the present study to investigate whether abdominal surgery prior to stressor exposure also promotes respective stress-induced spleen effects in the absence of any bite wounds. In line with our hypothesis, abdominal surgery prior to CSC induced splenomegaly, increased in vitro cell viability under basal and LPS conditions as well as the delta response to LPS (LPS – basal), and promoted the inability of isolated splenocytes to respond with a decreased cell viability to increasing concentrations of corticosterone following LPS-stimulation in vitro. Together with previous data, these findings demonstrate that physical injury, either in form of received bite wounds during stressor exposure or in form of abdominal surgery prior to stressor exposure, promotes the development of splenic immune activation and GC resistance. |
| Databáze: | OpenAIRE |
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