Citrus peel polymethoxyflavones, sudachitin and nobiletin, induce distinct cellular responses in human keratinocyte HaCaT cells
| Autor: | Keizo Yuasa, Saki Hirose, Mami Nishitani, Shogo Abe, Akihiko Tsuji, Ichiro Yoshida, Masao Tsukayama |
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| Rok vydání: | 2018 |
| Předmět: |
Keratinocytes
0301 basic medicine Citrus Poly ADP ribose polymerase Apoptosis Applied Microbiology and Biotechnology Biochemistry Nobiletin Cell Line Analytical Chemistry Structure-Activity Relationship 03 medical and health sciences chemistry.chemical_compound Drug Discovery Autophagy Humans Glycosides Propidium iodide Molecular Biology Cell Proliferation Flavonoids chemistry.chemical_classification Reactive oxygen species Organic Chemistry Biological activity General Medicine Flavones Cell biology HaCaT 030104 developmental biology chemistry Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 82:2064-2071 |
| ISSN: | 1347-6947 0916-8451 |
| Popis: | A variety of polyphenols have been isolated from plants, and their biological activities have been examined. Sudachitin (5,7,4′-trihydroxy-6,8,3′-trimethoxyflavone) is a polymethoxyflavone that is isolated from the peel of Citrus sudachi. Although we previously reported that sudachitin possesses an anti-inflammatory activity, its other biological activities are not yet understood. In this study, we report a novel biological activity of sudachitin, which selectively induced apoptosis in human keratinocyte HaCaT cells. Another polymethoxyflavone, nobiletin (5,6,7,8,3′,4′-hexamethoxyflavone), promoted autophagy but not apoptosis in HaCaT cells. On the other hand, 3′-demethoxysudachitin (5,7,4′-trihydroxy-6,8-dimethoxyflavone) failed to induce apoptosis and autophagy. These results show that three polymethoxyflavones have different effects on apoptosis and autophagy in HaCaT cells. Understanding the structure and biological activity of polymethoxyflavones may lead to the discovery of potential candidates for cancer drug development without significant toxic side effects. Abbreviations: ROS: reactive oxygen species; DMSO: dimethyl sulfoxide; MTT: 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; PARP: poly(ADP-ribose) polymerase; PI: propidium iodide; MAPK: mitogen-activated protein kinase. |
| Databáze: | OpenAIRE |
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