EDACO, a derivative of myricetin, inhibits the differentiation of Gaoyou duck embryonic osteoclasts in vitro
| Autor: | Yingxiao Fu, Wei Xue, J C Yuan, Yihui Wang, Xi-Shuai Tong, Baoding Tang, H G Dai, T T Zheng, Huang Yinjiu, Yingji Mao, Xianfu Liu, Chuang Li, Xuemin Sun, Dequn Niu, Zheng Gong |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Embryo
Nonmammalian 040301 veterinary sciences Osteoclasts Peripheral blood mononuclear cell 0403 veterinary science chemistry.chemical_compound Multinucleate Animals Receptor Cells Cultured Flavonoids biology Activator (genetics) 0402 animal and dairy science Acid phosphatase Cell Differentiation 04 agricultural and veterinary sciences General Medicine 040201 dairy & animal science Molecular biology In vitro Resorption Ducks chemistry biology.protein Animal Science and Zoology Myricetin Food Science |
| Zdroj: | British poultry science. 60(2) |
| ISSN: | 1466-1799 |
| Popis: | 1. This study determined the effects of (E)-3-(2-(4-(3-(2,4-dimethoxyphenyl)acryloyl)phenoxy)ethoxy)-5,7-dimethoxy-2-(3,4,5-trimethoxyphenyl)-4H-chromen-4-one (EDACO) on the differentiation of Gaoyou duck embryonic osteoclasts cultured in vitro. 2. Bone marrow mononuclear cells (BM-MNC) were collected from 23-d-old Gaoyou duck embryos and induced by macrophage colony-stimulating factor and receptor activator of nuclear factor κB ligand in the presence of EDACO at different concentrations (i.e. 10, 20, 40, 80 and 160 µM). Tartrate-resistant acid phosphatase (TRAP) staining and resorption ability determination were conducted. 3. Results suggested that EDACO suppressed the shaping of positive multinucleated cells and the number of TRAP-positive cells in the 20, 40, 80 and 160 μM EDACO groups was significantly decreased (P < 0.05 or P < 0.01). Besides, the absorption activity of differentiated duck embryonic osteoclasts was significantly inhibited (P < 0.05) in both 80 and 160 μM EDACO groups. 4. Overall, EDACO can inhibit the differentiation of BM-MNC into mature osteoclasts in duck embryos.1. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|