Dietary Pseudopurpurin Improves Bone Geometry Architecture and Metabolism in Red-Bone Guishan Goats

Autor: Tie-Suo Han, Guowen Liu, Changrong Ge, Shizheng Gao, Chen-Chen Wu, Jianguo Wang, Peng Li, Xiaobing Li, Zhe Wang
Rok vydání: 2012
Předmět:
Anatomy and Physiology
Magnetic Resonance Spectroscopy
Bone density
Agricultural Biotechnology
lcsh:Medicine
Veterinary Anatomy and Physiology
Anthraquinones
Biochemistry
Mass Spectrometry
Analytical Chemistry
Absorptiometry
Photon
Femur
lcsh:Science
Musculoskeletal System
Chromatography
High Pressure Liquid
Animal Management
Bone mineral
Multidisciplinary
Molecular Structure
biology
Goats
Agriculture
Anatomy
Biomechanical Phenomena
Chemistry
medicine.anatomical_structure
Osteocalcin
Alkaline phosphatase
Research Article
Biotechnology
Veterinary Medicine
China
medicine.medical_specialty
Bone and Mineral Metabolism
Animal Types
Real-Time Polymerase Chain Reaction
Osteoprotegerin
Internal medicine
Chemical Biology
medicine
Animals
Rats
Wistar
Bone
Biology
DNA Primers
Analysis of Variance
Plant Extracts
Organic Chemistry
Rubia
lcsh:R
Metabolism
Rats
Endocrinology
biology.protein
Veterinary Science
lcsh:Q
Bone marrow
Zoology
Zdroj: PLoS ONE, Vol 7, Iss 5, p e37469 (2012)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0037469
Popis: Red-colored bones were found initially in some Guishan goats in the 1980s, and they were designated red-boned goats. However, it is not understood what causes the red color in the bone, or whether the red material changes the bone geometry, architecture, and metabolism of red-boned goats. Pseudopurpurin was identified in the red-colored material of the bone in red-boned goats by high-performance liquid chromatography-electrospray ionization-mass spetrometry and nuclear magnetic resonance analysis. Pseudopurpurin is one of the main constituents of Rubia cordifolia L, which is eaten by the goats. The assessment of the mechanical properties and micro-computed tomography showed that the red-boned goats displayed an increase in the trabecular volume fraction, trabecular thickness, and the number of trabeculae in the distal femur. The mean thickness, inner perimeter, outer perimeter, and area of the femoral diaphysis were also increased. In addition, the trabecular separation and structure model index of the distal femur were decreased, but the bone mineral density of the whole femur and the mechanical properties of the femoral diaphysis were enhanced in the red-boned goats. Meanwhile, expression of alkaline phosphatase and osteocalcin mRNA was higher, and the ratio of the receptor activator of the nuclear factor kappa B ligand to osteoprotegerin was markedly lower in the bone marrow of the red-boned goats compared with common goats. To confirm further the effect of pseudopurpurin on bone geometry, architecture, and metabolism, Wistar rats were fed diets to which pseudopurpurin was added for 5 months. Similar changes were observed in the femurs of the treated rats. The above results demonstrate that pseudopurpurin has a close affinity with the mineral salts of bone, and consequently a high level of mineral salts in the bone cause an improvement in bone strength and an enhancement in the structure and metabolic functions of the bone.
Databáze: OpenAIRE
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