Prenatal Exposure to Urban Air Nanoparticles in Mice Causes Altered Neuronal Differentiation and Depression-Like Responses
| Autor: | Nahoko Iwata, Sean C. Godar, Marco Bortolato, Todd E. Morgan, Rob McConnell, David A. Davis, Caleb E. Finch, Jean C. Shih, Constantinos Sioutas, Thomas Sander, Payam Pakbin, Kiros Berhane |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Pathology Cellular differentiation lcsh:Medicine Hippocampus Pilot Projects Developmental and Pediatric Neurology 010501 environmental sciences Toxicology 01 natural sciences Mice Behavioral Neuroscience Engineering 0302 clinical medicine Pregnancy 11. Sustainability lcsh:Science Cells Cultured Neurons Psychiatry Air Pollutants Multidisciplinary integumentary system Obstetrics and Gynecology Cell Differentiation Pollution Anxiety Disorders Chemistry Mental Health medicine.anatomical_structure Neurology Cerebral cortex Prenatal Exposure Delayed Effects Medicine Gestation Female Research Article Neurotoxicology Veterinary Medicine medicine.medical_specialty Environmental Engineering Neurite Neurogenesis Blotting Western Air Quality Veterinary Neurology Andrology 03 medical and health sciences Glutamatergic Developmental Neuroscience Neurites medicine Animals Humans Environmental Chemistry Cities Maze Learning Biology 0105 earth and related environmental sciences Depressive Disorder business.industry lcsh:R JNK Mitogen-Activated Protein Kinases medicine.disease Mice Inbred C57BL Animals Newborn 13. Climate action Atmospheric Chemistry Nanoparticles Particulate Matter Veterinary Science lcsh:Q Neuron business 030217 neurology & neurosurgery Neuroscience |
| Zdroj: | PLoS ONE, Vol 8, Iss 5, p e64128 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Emerging evidence suggests that excessive exposure to traffic-derived air pollution during pregnancy may increase the vulnerability to neurodevelopmental alterations that underlie a broad array of neuropsychiatric disorders. We present a mouse model for prenatal exposure to urban freeway nanoparticulate matter (nPM). In prior studies, we developed a model for adult rodent exposure to re-aerosolized urban nPM which caused inflammatory brain responses with altered neuronal glutamatergic functions. nPMs are collected continuously for one month from a local freeway and stored as an aqueous suspension, prior to re-aerosolization for exposure of mice under controlled dose and duration. This paradigm was used for a pilot study of prenatal nPM impact on neonatal neurons and adult behaviors. Adult C57BL/6J female mice were exposed to re-aerosolized nPM (350 µg/m(3)) or control filtered ambient air for 10 weeks (3×5 hour exposures per week), encompassing gestation and oocyte maturation prior to mating. Prenatal nPM did not alter litter size, pup weight, or postnatal growth. Neonatal cerebral cortex neurons at 24 hours in vitro showed impaired differentiation, with 50% reduction of stage 3 neurons with long neurites and correspondingly more undifferentiated neurons at Stages 0 and 1. Neuron number after 24 hours of culture was not altered by prenatal nPM exposure. Addition of exogenous nPM (2 µg/ml) to the cultures impaired pyramidal neuron Stage 3 differentiation by 60%. Adult males showed increased depression-like responses in the tail-suspension test, but not anxiety-related behaviors. These pilot data suggest that prenatal exposure to nPM can alter neuronal differentiation with gender-specific behavioral sequelae that may be relevant to human prenatal exposure to urban vehicular aerosols. |
| Databáze: | OpenAIRE |
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