Protective effects of Gamma Oryzanol on distant organs after kidney ischemia-reperfusion in rats: A focus on liver protection
| Autor: | Shadi Aghajani, Yasin Bagheri, Mahla Hosseinzadeh, Farid Hoshmandan, Sepideh Zununi Vahed, Abdollah Abdollahpour |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty 2019-20 coronavirus outbreak Coronavirus disease 2019 (COVID-19) Health Toxicology and Mutagenesis Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) 030232 urology & nephrology Toxicology Kidney Protective Agents 03 medical and health sciences 0302 clinical medicine Medicine Animals Humans Rats Wistar Renal ischemia Phenylpropionates business.industry Acute kidney injury GAMMA ORYZANOL General Medicine Acute Kidney Injury medicine.disease Rats Multi organ dysfunction Disease Models Animal 030104 developmental biology Reperfusion Injury business Hepatic dysfunction |
| Zdroj: | Humanexperimental toxicology. 40(6) |
| ISSN: | 1477-0903 |
| Popis: | Background:Acute kidney injury (AKI) is the main clinical concern resulted from ischemia-reperfusion injury (IRI). Ample clinical data indicates that AKI is associated with distant organ dysfunctions and poor patients’ outcomes. Oxidative stress and inflammation have a critical role in the pathogenesis of organ injuries following IRI. The objectives of this study were to determine the impact of Gamma Oryzanol (GO), extracted from rice bran oil, on distant organs in rats after IRI.Methods:Twelve out of 24 Wistar rats were treated by one dosage of GO (100mg/kg) 1 h before I/R induction through both oral gavage and intraperitoneal injection. Then, the AKI model rats were induced by IRI. Oxidative stress and antioxidant protein levels were assessed in the brain, heart, and liver tissues in the experimental groups. Furthermore, the effects of GO on IRI-induced liver dysfunction, apoptosis, and inflammation were measured by Western blot.Results:GO pretreatment could significantly restore the levels and activity of antioxidant proteins in the brain, heart, and liver tissues (P < 0.05). Moreover, GO pretreatment could decrease the inflammatory cytokine (IL-1, IL-6, and TNF-α) in the liver (P < 0.01). By reducing Bax/Bcl-2 ratio and down-regulating caspase-3, GO could significantly diminish apoptosis in the liver tissue after the kidney I/R (P < 0.01). Additionally, GO could significantly diminish the deterioration of liver function in the kidney I/R model.Conclusion:GO protects distant organs against renal IRI-induced oxidative stress. Furthermore, it ameliorates liver function and remarkably exerts anti-oxidative, anti-inflammatory, and anti-apoptotic roles in the liver as an important detoxifying organ. |
| Databáze: | OpenAIRE |
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