Comparison of Proteins Involved in Pilus Synthesis and Mating Pair Stabilization from the Related Plasmids F and R100-1: Insights into the Mechanism of Conjugation
| Autor: | William A. Klimke, Laura S. Frost, Jan Manchak, Karen G. Anthony |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
DNA
Bacterial R Factors Molecular Sequence Data Mutagenesis (molecular biology technique) Biology Microbiology Pilus F Factor Complete sequence Plasmid Bacterial Proteins Molecular Biology Gene Genetics F-factor Base Sequence Genetic Complementation Test Genetic Variation Pili Sex Relaxosome Genes Bacterial Mutagenesis Conjugation Genetic Fimbriae Bacterial Plasmids and Transposons Heteroduplex |
| Zdroj: | ResearcherID |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.181.17.5149-5159.1999 |
| Popis: | F and R100-1 are closely related, derepressed, conjugative plasmids from the IncFI and IncFII incompatibility groups, respectively. Heteroduplex mapping and genetic analyses have revealed that the transfer regions are extremely similar between the two plasmids. Plasmid specificity can occur at the level of relaxosome formation, regulation, and surface exclusion between the two transfer systems. There are also differences in pilus serology, pilus-specific phage sensitivity, and requirements for OmpA and lipopolysaccharide components in the recipient cell. These phenotypic differences were exploited in this study to yield new information about the mechanism of pilus synthesis, mating pair stabilization, and surface and/or entry exclusion, which are collectively involved in mating pair formation (Mpf). The sequence of the remainder of the transfer region of R100-1 ( trbA to traS ) has been completed, and the complete sequence is compared to that of F. The differences between the two transfer regions include insertions and deletions, gene duplications, and mosaicism within genes, although the genes essential for Mpf are conserved in both plasmids. F + cells carrying defined mutations in each of the Mpf genes were complemented with the homologous genes from R100-1. Our results indicate that the specificity in recipient cell recognition and entry exclusion are mediated by TraN and TraG, respectively, and not by the pilus. |
| Databáze: | OpenAIRE |
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