Different Types of Urinary Steroid Profiling Obtained by High-Performance Liquid Chromatography and Gas Chromatography-Mass Spectrometry in Patients with Adrenocortical Carcinoma

Autor: E. G. Strel’nikova, L I Velikanova, E A Kukhianidze, A. L. Moskvin, A A Lisitsin, E A Fedorov, E A Bessonova, I V Sablin, Z R Shafigullina, N. S. Krivokhizhina, N V Vorokhobina, L M Krasnov, K Grigoryan
Rok vydání: 2016
Předmět:
Adult
Male
Cancer Research
medicine.medical_specialty
Endocrinology
Diabetes and Metabolism
Cortodoxone
Dehydroepiandrosterone
030209 endocrinology & metabolism
High-performance liquid chromatography
Gas Chromatography-Mass Spectrometry
Adrenocortical adenoma
Diagnosis
Differential
Young Adult
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
Internal medicine
Adrenocortical Carcinoma
medicine
Humans
Adrenocortical carcinoma
Steroid 11-beta-hydroxylase
Cushing Syndrome
Chromatography
High Pressure Liquid
Tetrahydrocorticosterone
Endocrine and Autonomic Systems
Chemistry
Adrenal cortex
Middle Aged
medicine.disease
Adrenal Cortex Neoplasms
stomatognathic diseases
Glucocorticoid secretion
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Adrenocortical Adenoma
Steroid 11-beta-Hydroxylase
Female
Steroids
Steroid 21-Hydroxylase
human activities
Zdroj: Europe PubMed Central
ISSN: 1868-8500
1868-8497
DOI: 10.1007/s12672-016-0267-0
Popis: Urinary steroid profiling (USP) was studied using high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) methods in 108 patients with adrenocortical adenoma (ACA) and in 31 patients with adrenocortical carcinoma (ACC). Thirteen ACC and Cushing's syndrome (ACC-CS) patients had two types of USP as well as 18 ACC patients without hypercortisolism. These four types differed by androgen and glucocorticoid secretion of the adrenal cortex. Fifteen main ACC features were observed by GC-MS. Urinary excretion of dehydroepiandrosterone (DHEA) was increased in 67.7 % of ACC patients and tetrahydro-11-deoxycortisol (THS) in 74.2 %. By combination of the following parameters: THS >900 μg/24 h and/or DHEA >1500 μg/24 h with ratios of 3α,16,20-pregnentriol/3β,16,20-pregnentriol (3α,16,20dP3/3β,16,20dP3) less than 6.0 and 3α,17,20dP3/3β,17,20dP3 less than 9.0 and the detection of "non-classical" 5-en-pregnens, not found in ACA and healthy persons, 100 % sensitivity and specificity of ACC and ACA differential diagnosis were achieved. Features of 21-hydroxylase and 11β-hydroxylase deficiency were observed by GC-MS in 32.2 and 61.3 % of the ACC patients, respectively. Additional features for ACC-CS diagnostic were increased urinary excretion of 6β-hydroxycortisol, 18-hydroxycorticosterone, the sum (UFF + UFE) obtained by HPLC, tetrahydrocorticosterone, and the sum (THF + THE + allo-THF) obtained by GC-MS.
Databáze: OpenAIRE
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