A gas chromatographic/mass spectrometric assay for prenylamine suitable for pharmacokinetic studies of the racemate and the enantiomers
| Autor: | E. K. Schmidt, W. D. Paar, H. J. Dengler, G. E. von Unruh |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Chromatography
Chemistry Prenylamine Kinetics Biological Availability Stereoisomerism Decane Biochemistry Gas Chromatography-Mass Spectrometry chemistry.chemical_compound Pharmacokinetics medicine Mass spectrum Humans Molecular Medicine Enantiomer Gas chromatography–mass spectrometry Acetonitrile Spectroscopy medicine.drug |
| Zdroj: | Biological Mass Spectrometry. 21:103-108 |
| ISSN: | 1096-9888 1052-9306 |
| Popis: | A sensitive assay for prenylamine and dideuteroprenylamine (racemic or pseudo-racemate) has been developed and used in human pharmacokinetic studies. Plasma levels of prenylamine could be measured up to 50 h after a single oral therapeutic dose. The extracted drug was derivatized with pentafluoropropionic anhydride in acetonitrile. The dried samples were reconstituted in decane; an aliquot was injected into a fused-silica capillary in a cooled on-column injector. The base peaks in the electron impact mass spectra of the compounds--derived by loss of a benzyl radical--at m/z 384, 386 and 390 were measured for prenylamine, (D2)-prenylamine and the internal standard hexahydroprenylamine, respectively. The sensitivity of this assay--limit of detection 0.2 ng ml-1 plasma with a signal-to-noise ratio of 5:1--allowed measurement of the kinetics of the racemate and of both stereoisomers for the first time. In man, the (+)-isomer was eliminated considerably faster than the (-)-prenylamine; the area under the plasma concentration time curve (AUC) of the (+)-isomer was only about 1/4 of the AUC of (-)-prenylamine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text