Design of a retroviral-mediated ecdysone-inducible system and its application to the expression profiling of the PTEN tumor suppressor
| Autor: | Michael Wigler, Linda Rodgers, Gregory J. Hannon, Javor P. Stolarov, Kenneth Chang, Vivek Mittal, Andrew Reiner |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Ecdysone
Transcription Genetic Genetic Vectors chemistry.chemical_compound Complementary DNA Tumor Cells Cultured Humans PTEN Genes Tumor Suppressor Phosphatidylinositol Gene Multidisciplinary biology Kinase Microarray analysis techniques Gene Expression Profiling Tumor Suppressor Proteins PTEN Phosphohydrolase Biological Sciences Flow Cytometry Phosphoric Monoester Hydrolases Gene expression profiling Retroviridae chemistry Cancer research biology.protein DNA microarray |
| Zdroj: | Proceedings of the National Academy of Sciences. 98:13043-13048 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.221450598 |
| Popis: | We have engineered the ecdysone-inducible mammalian expression system for general retroviral delivery to cultured mammalian cells. We inducibly expressed PTEN in the glioblastoma cell line, U87MG, lacking this gene. Because nearly all cells are recruited on induction, we find both up- and down-regulated genes by cDNA microarray analysis. The changes we see are similar to those observed after treatment with LY294002, an inhibitor of phosphatidylinositol 3-OH kinase, fully consistent with the model that PTEN antagonizes phosphatidylinositol 3-OH kinase. Both treatments result in suppressed expression of the transforming growth factor (TGF)-β gene and the genes of the cholesterol biosynthesis pathway. Our results illustrate the power of using a fully inducible expression system in conjunction with cDNA microarray analysis for exploring gene function. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|