siRNA biogenesis and advances in topically applied dsRNA for controlling virus infections in tomato plants
| Autor: | Natália Lucinda, Tatsuya Nagata, Alice K. Inoue-Nagata, João Marcos Fagundes Silva, Erich Y.T. Nakasu, Camila M. Rego-Machado |
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| Přispěvatelé: | CAMILA M. REGO-MACHADO, UNIVERSIDADE DE BRASÍLIA, ERICH YUKIO TEMPEL NAKASU, CNPH, JOÃO M. F. SILVA, UNIVERSIDADE DE BRASÍLIA, NATÁLIA LUCINDA, UNIVERSITY OF FLORIDA, TATSUYA NAGATA, UNIVERSIDADE DE BRASÍLIA, ALICE KAZUKO INOUE NAGATA, CNPH. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine viruses Science Potyvirus 01 natural sciences Article Virus 03 medical and health sciences Solanum lycopersicum Mosaic Viruses RNA interference Tomate Plant virus Tobacco Tomato mosaic virus RNA Small Interfering Double-stranded RNA Plant Diseases RNA Double-Stranded Multidisciplinary biology Biological techniques Tobamovirus Begomovirus fungi Vírus RNA food and beverages biology.organism_classification Virology RNA silencing 030104 developmental biology Potato virus Y Virus Diseases Medicine Plant sciences Biotechnology 010606 plant biology & botany |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020) Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | A non-transgenic approach based on RNA interference was employed to induce protection against tomato mosaic virus (ToMV) infection in tomato plants. dsRNA molecules targeting the cp gene of ToMV were topically applied on plants prior to virus inoculation. Protection was dose-dependent and sequence-specific. While no protection was achieved when 0–16 µg dsRNA were used, maximum rates of resistance (60 and 63%) were observed in doses of 200 and 400 µg/plant, respectively. Similar rates were also obtained against potato virus Y when targeting its cp gene. The protection was quickly activated upon dsRNA application and lasted for up to 4 days. In contrast, no detectable antiviral response was triggered by the dsRNA from a begomovirus genome, suggesting the method is not effective against phloem-limited DNA viruses. Deep sequencing was performed to analyze the biogenesis of siRNA populations. Although long-dsRNA remained in the treated leaves for at least 10 days, its systemic movement was not observed. Conversely, dsRNA-derived siRNA populations (mainly 21- and 22-nt) were detected in non-treated leaves, which indicates endogenous processing and transport through the plant. Altogether, this study provides critical information for the development of novel tools against plant viruses; strengths and limitations inherent to the systems are discussed. |
| Databáze: | OpenAIRE |
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