Immobilized Chemokine Fields and Soluble Chemokine Gradients Cooperatively Shape Migration Patterns of Dendritic Cells
| Autor: | Tim Lämmermann, Julien Polleux, Gerold Schuler, Manfred B. Lutz, Markus Bruckner, Reinhold Förster, Daniel F. Legler, Joachim P. Spatz, Kathrin Schumann, Michael Sixt, Lydia Sorokin |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Chemokine
endocrine system Integrins Receptors CCR7 PROTEINS Surface Properties Integrin Fluoroimmunoassay Immunology C-C chemokine receptor type 7 Article 03 medical and health sciences Mice 0302 clinical medicine Cell Movement ddc:570 Animals Immunology and Allergy MOLIMMUNO Cell adhesion CXCL16 Cells Cultured 030304 developmental biology Mice Knockout 0303 health sciences biology Chemokine CCL21 CCL19 Dendritic Cells biochemical phenomena metabolism and nutrition Cells Immobilized Recombinant Proteins Cell biology Mice Inbred C57BL Reticulin Infectious Diseases Solubility biology.protein bacteria Chemokine CCL19 Lymph Nodes CCL25 Chemokines 030215 immunology CCL21 |
| Zdroj: | Immunity |
| ISSN: | 1074-7613 |
| DOI: | 10.1016/j.immuni.2010.04.017 |
| Popis: | SummaryChemokines orchestrate immune cell trafficking by eliciting either directed or random migration and by activating integrins in order to induce cell adhesion. Analyzing dendritic cell (DC) migration, we showed that these distinct cellular responses depended on the mode of chemokine presentation within tissues. The surface-immobilized form of the chemokine CCL21, the heparan sulfate-anchoring ligand of the CC-chemokine receptor 7 (CCR7), caused random movement of DCs that was confined to the chemokine-presenting surface because it triggered integrin-mediated adhesion. Upon direct contact with CCL21, DCs truncated the anchoring residues of CCL21, thereby releasing it from the solid phase. Soluble CCL21 functionally resembles the second CCR7 ligand, CCL19, which lacks anchoring residues and forms soluble gradients. Both soluble CCR7 ligands triggered chemotactic movement, but not surface adhesion. Adhesive random migration and directional steering cooperate to produce dynamic but spatially restricted locomotion patterns closely resembling the cellular dynamics observed in secondary lymphoid organs.PaperClip |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|