Handling of the Cotton Rat in Studies for the Pre-clinical Evaluation of Oncolytic Viruses
| Autor: | Breanne P Cuddington, Meghan L. Verschoor, Karen L. Mossman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
General Chemical Engineering
Context (language use) Bone Neoplasms General Biochemistry Genetics and Molecular Biology Immune system Stress Physiological Virology medicine Animals Cotton rat Sigmodontinae Oncolytic Virotherapy Mammary tumor Osteosarcoma biology General Immunology and Microbiology business.industry General Neuroscience Mammary Neoplasms Experimental Neoplasms Experimental medicine.disease biology.organism_classification Oncolytic virus Rats Clinical trial Disease Models Animal Oncolytic Viruses Immunology Cancer research Female business Clinical evaluation |
| Popis: | Oncolytic viruses are a novel anticancer therapy with the ability to target tumor cells, while leaving healthy cells intact. For this strategy to be successful, recent studies have shown that involvement of the host immune system is essential. Therefore, oncolytic virotherapy should be evaluated within the context of an immunocompetent model. Furthermore, the study of antitumor therapies in tolerized animal models may better recapitulate results seen in clinical trials. Cotton rats, commonly used to study respiratory viruses, are an attractive model to study oncolytic virotherapy as syngeneic models of mammary carcinoma and osteosarcoma are well established. However, there is a lack of published information on the proper handling procedure for these highly excitable rodents. The handling and capture approach outlined minimizes animal stress to facilitate experimentation. This technique hinges upon the ability of the researcher to keep calm during handling and perform procedures in a timely fashion. Finally, we describe how to prepare cotton rat mammary tumor cells for consistent subcutaneous tumor formation, and how to perform intratumoral and intraperitoneal injections. These methods can be applied to a wide range of studies furthering the development of the cotton rat as a relevant pre-clinical model to study antitumor therapy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|