The interval between a septic stimulus and hypoxia/reoxygenation affects cytokine elaboration by murine peritoneal macrophages
| Autor: | Victor F. Garcia, Gajra Arya |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Lipopolysaccharides
medicine.medical_specialty Time Factors Lipopolysaccharide medicine.medical_treatment In Vitro Techniques Nitric Oxide Dinoprostone Nitric oxide chemistry.chemical_compound Mice In vivo Internal medicine medicine Animals Hypoxia Analysis of Variance business.industry Tumor Necrosis Factor-alpha General Medicine Hypoxia (medical) Macrophage Activation Shock Septic Pathophysiology Disease Models Animal Cytokine Endocrinology chemistry Pediatrics Perinatology and Child Health Macrophages Peritoneal Mice Inbred CBA Cytokines Surgery Tumor necrosis factor alpha medicine.symptom business Prostaglandin E |
| Zdroj: | Journal of pediatric surgery. 31(11) |
| ISSN: | 0022-3468 |
| Popis: | The critically ill patient is commonly exposed to various physiological insults. The authors have previously shown that in vivo hypoxia/reoxygenation (H/R) alters the pattern of cytokines elaborated by murine peritoneal macrophages given a septic stimulus. In this study they sought to determine whether the interval between a septic stimulus and H/R affected the release of inflammatory mediators by macrophages. Adult CBA-strain mice were injected intraperitoneally with 10 micrograms of lipopolysaccharide (LPS). On day 0, 1, 2, 3, 4, or 5 after LPS injection, animals were exposed to 16 hours of hypoxia followed by 2 hours of reoxygenation. Harvested peritoneal macrophages were restimulated in vitro with 2.5 micrograms/mL LPS or left unstimulated. Culture supernatants collected at 2, 4, 6, 8, 10, and 12 hours after LPS injection were assayed for tumor necrosis factor (TNF), prostaglandin E2 (PGE2), and nitric oxide (NO) production. Macrophage-derived mediator production peaked when H/R occurred 3 days following LPS injection (P.05). These data suggest that the interval between sepsis and subsequent H/R influences the pattern of cytokines elaborated by peritoneal macrophages given a septic stimulus. |
| Databáze: | OpenAIRE |
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