Campomanesia xanthocarpa (Mart.) O. Berg essential oil induces antileishmanial activity and remodeling of the cytoplasm organelles
| Autor: | Áquila Carolina Fernandes Herculano Ramos-Milaré, Thaís Gomes Verzignassi Silveira, Rosangela Rumi Sugauara, Izabel Galhardo Demarchi, Celso Vataru Nakamura, José Eduardo Gonçalves, Fabiana Borges Padilha Ferreira, Carla Maria Mariano Fernandez, Zilda Cristiani Gazim, Maria Valdrinez Campana Lonardoni, Danielle Lazarin-Bidóia |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
010405 organic chemistry
Chemistry Organic Chemistry Leishmaniasis Plant Science Sesquiterpene medicine.disease 01 natural sciences Biochemistry 0104 chemical sciences Analytical Chemistry law.invention Microbiology 010404 medicinal & biomolecular chemistry chemistry.chemical_compound Cutaneous leishmaniasis law medicine Parasite hosting Cytotoxicity Amastigote IC50 Essential oil |
| DOI: | 10.6084/m9.figshare.13032691 |
| Popis: | Leishmaniasis is a neglected disease that affects millions of people worldwide. This study aimed to analyze antileishmanial activity of Campomanesia xanthocarpa leaf essential oil (EO) on promastigote and amastigote forms of Leishmania amazonensis, cytotoxicity in murine macrophages and sheep erythrocytes. The essential oil (EO) was analyzed by gas chromatography/mass spectrophotometry. The main and most abundant compounds were sesquiterpene hydrocarbons (71.22%) such as trans-caryophyllene (7.87%), bicyclogermacrene (11.28%), and δ-cadinene (8.34%). The IC50 for promastigote and amastigote forms of L. amazonensis was 70 µg mL−1 and 6 µg mL−1, respectively. C. xanthocarpa EO was not cytotoxic for murine macrophages (CC50 1860 µg mL−1) and sheep erythrocytes (1.5%), presenting high selectivity index for protozoan (310). C. xanthocarpa EO induced effects on the morphology and ultrastructure of this parasite. The high activity for intracellular amastigote forms, low toxicity to murine macrophages, and erythrocytes, suggest that C. xanthocarpa EO is promising for the treatment of leishmaniasis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|