Genomic signatures of Lake Erie bacteria suggest interaction in the Microcystis phycosphere

Autor: Morgan M. Steffen, Naomi E. Gilbert, Dominique J. Lockwood, Morgan R. Smith, Louie L. Wurch, Zachary P. Zimmerman, Guadalupe Reynoso, Shannon R. Farnan, Steven W. Wilhelm, Malia I. Gardner, Katherine E. Crider, Lucy K. Utz, Victoria Mendoza, Alexa K. Hoke, Alison C. Poole, Grant J. Brennan, Isabella R. Becker
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Cyanobacteria
Marine and Aquatic Sciences
Fresh Water
Pathology and Laboratory Medicine
Carbon utilization
Medicine and Health Sciences
Urea
Phylogeny
Multidisciplinary
biology
Bacterial Genomics
Organic Compounds
Microbiota
Microbial Genetics
High-Throughput Nucleotide Sequencing
Quorum Sensing
Eukaryota
Enterobacter
Genomics
Plants
Marine Bacteria
Plankton
Bacterial Pathogens
Chemistry
Medical Microbiology
Physical Sciences
Medicine
Pathogens
Bloom
Oxidation-Reduction
Signal Transduction
Research Article
Freshwater Environments
DNA
Bacterial
Microcystis
Algae
Nitrogen
Harmful Algal Bloom
Science
Microbial Genomics
Algal bloom
Microbiology
Botany
Genetics
Bacterial Genetics
Animals
Microbial Pathogens
Bacteria
Organic Chemistry
Ecology and Environmental Sciences
fungi
Organisms
Chemical Compounds
Biology and Life Sciences
Aquatic Environments
Bacteriology
biology.organism_classification
Invertebrates
Carbon
United States
Lakes
Phytoplankton
Earth Sciences
Metagenome
Zoology
Genome
Bacterial
Zdroj: PLoS ONE, Vol 16, Iss 9, p e0257017 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: Microbial interactions in harmful algal bloom (HAB) communities have been examined in marine systems, but are poorly studied in fresh waters. To investigate HAB-microbe interactions, we isolated bacteria with close associations to bloom-forming cyanobacteria, Microcystis spp., during a 2017 bloom in the western basin of Lake Erie. The genomes of five isolates (Exiguobacterium sp. JMULE1, Enterobacter sp. JMULE2, Deinococcus sp. JMULE3, Paenibacillus sp. JMULE4, and Acidovorax sp. JMULE5.) were sequenced on a PacBio Sequel system. These genomes ranged in size from 3.1 Mbp (Exiguobacterium sp. JMULE1) to 5.7 Mbp (Enterobacter sp. JMULE2). The genomes were analyzed for genes relating to critical metabolic functions, including nitrogen reduction and carbon utilization. All five of the sequenced genomes contained genes that could be used in potential signaling and nutrient exchange between the bacteria and cyanobacteria such as Microcystis. Gene expression signatures of algal-derived carbon utilization for two isolates were identified in Microcystis blooms in Lake Erie and Lake Tai (Taihu) at low levels, suggesting these organisms are active and may have a functional role during Microcystis blooms in aggregates, but were largely missing from whole water samples. These findings build on the growing evidence that the bacterial microbiome associated with bloom-forming algae have the functional potential to contribute to nutrient exchange within bloom communities and interact with important bloom formers like Microcystis.
Databáze: OpenAIRE
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