Differentiation in vitro of Embryonic Cartilage and Bone in a Chemically-Defined Medium
| Autor: | Charity Waymouth, L. Whittington Gorham |
|---|---|
| Rok vydání: | 1965 |
| Předmět: |
Photomicrography
Embryo Nonmammalian Histology Cellular differentiation In Vitro Techniques Bone and Bones General Biochemistry Genetics and Molecular Biology Tissue Culture Techniques Andrology Mice Tissue culture Osteogenesis medicine Animals Bovine serum albumin biology Research Cartilage Cell Differentiation Embryo Embryo Mammalian In vitro Culture Media Chemically defined medium medicine.anatomical_structure Immunology biology.protein |
| Zdroj: | Experimental Biology and Medicine. 119:287-290 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-119-30160 |
| Popis: | Summary and conclusions1. Fell first reported osteogenesis in chick embryo limb buds cultured in vitro in a plasma clot in 1928. Since that time several attempts to repeat her work using a completely-defined chemical medium have failed. Except in one instance, success was achieved only when biologic supplements, e.g., embryo extract or bovine serum albumin, were added to the medium. 2. This communication reports differentiation of embryonic cartilage and bone of the mouse in vitro using a new, completely-defined chemical medium (MAB87/3) which is the simplest thus far reported. Biologic supplements were not necessary. 3. The 14-day embryo limb rudiment develops a rim of perichondrial bone after 5 days of culture in this medium. A further increase in amount of new bone was visible after 12 days of culture. |
| Databáze: | OpenAIRE |
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