Synthesis of Isotopically Labelled, Spin-Isolated Tyrosine and Phenylalanine for Protein NMR Applications
| Autor: | Munia Sowaileh, Jitendra Das, Charalampos G. Kalodimos, Yixin Cui, Zoran Rankovic, Brandon Young, P. Jake Slavish, Paolo Rossi |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Magnetic Resonance Spectroscopy Molecular Structure Chemistry Phenylalanine Organic Chemistry Proteins Sequence (biology) Nuclear magnetic resonance spectroscopy Biochemistry Combinatorial chemistry Article Amino acid Isotope Labeling Molecule Tyrosine Physical and Theoretical Chemistry Signal intensity Amino Acids Deoxygenation |
| Zdroj: | Org Lett |
| Popis: | Isotopically labeled amino acids are widely used to study the structure and dynamics of proteins by NMR. Herein we describe a facile, gram-scale synthesis of compounds 1b and 2b under standard laboratory conditions from the common intermediate 7. 2b is obtained via simple deprotection, while 1b is accessed through a reductive deoxygenation/deuteration sequence and deprotection. 1b and 2b provide improved signal intensity using lower amounts of labeled precursor and are alternatives to existing labeling approaches. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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