The Genomic and Phenotypic Diversity of Schizosaccharomyces pombe
| Autor: | Francesc Xavier Marsellach, Bartlomiej Tomiczek, Jared T. Simpson, Tobias Mourier, Francois Balloux, Kirsten McLay, Jürg Bähler, Sophie R. Atkinson, Daniel C. Jeffares, Theodora C. Sideri, Charalampos Rallis, Laurent van Trigt, Nikolas Maniatis, Melanie Febrer, Zamin Iqbal, Josephine E. E. U. Hellberg, Adrien Rieux, Richard Durbin, Tammy M. K. Cheng, William Brown, Rodrigo Pracana, Malte Thodberg, Anatole Chessel, Doug Speed, Leanne Bischof, Martin Převorovský, David J. Balding, Michael Mülleder, Danny A. Bitton, Markus Ralser, Rafael E. Carazo Salas, Brendan D. O'Fallon, Jacqueline Hayles, Sendu Bala, Nizar Drou, Linda Jeffery, Winston Lau, Jonathan L.D. Lawson, Garrett Hellenthal, Thomas M. Keane, Juan Juan Li, Sandra Codlin |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Genetics
Genome evolution Genetic diversity biology Genotype Population genetics Genetic Variation Genomics biology.organism_classification Polymorphism Single Nucleotide Article Phenotype Genetic variation Schizosaccharomyces pombe Schizosaccharomyces Humans Genome Fungal Genetic association Genome-Wide Association Study |
| Popis: | Natural variation within species reveals aspects of genome evolution and function. The fission yeast Schizosaccharomyces pombe is an important model for eukaryotic biology, but researchers typically use one standard laboratory strain. To extend the usefulness of this model, we surveyed the genomic and phenotypic variation in 161 natural isolates. We sequenced the genomes of all strains, finding moderate genetic diversity (π = 3 × 10(-3) substitutions/site) and weak global population structure. We estimate that dispersal of S. pombe began during human antiquity (∼340 BCE), and ancestors of these strains reached the Americas at ∼1623 CE. We quantified 74 traits, finding substantial heritable phenotypic diversity. We conducted 223 genome-wide association studies, with 89 traits showing at least one association. The most significant variant for each trait explained 22% of the phenotypic variance on average, with indels having larger effects than SNPs. This analysis represents a rich resource to examine genotype-phenotype relationships in a tractable model. |
| Databáze: | OpenAIRE |
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