Functional Immobilization of a Biofilm-Releasing Glycoside Hydrolase Dispersin B on Magnetic Nanoparticles
| Autor: | Zeng Kai, Ruoyu Wang, Zisong Zhao, Zewen Liu, Weihua Xu, Junhui Guo, Yue Xia, Hao Xie |
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| Rok vydání: | 2021 |
| Předmět: |
Chemistry
Biofilm Bioengineering General Medicine Silicon Dioxide medicine.disease_cause Applied Microbiology and Biotechnology Biochemistry Combinatorial chemistry Staphylococcus aureus medicine Zeta potential Magnetic nanoparticles Glycoside hydrolase Fourier transform infrared spectroscopy Dispersin B Molecular Biology Escherichia coli Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. 194:737-747 |
| ISSN: | 1559-0291 0273-2289 |
| DOI: | 10.1007/s12010-021-03673-y |
| Popis: | Dispersin B (DspB) is a member of glycoside hydrolase family 20 (GH20) and catalyzes degradation of biofilms forming by pathogenic bacteria such as Staphylococcus aureus. Magnetoreceptor (MagR) is a magnetic protein that can be used as a fusion partner for functionally immobilizing proteins on magnetic surfaces. In the present study, a recombinant protein DspB-MagR was constructed by fusing MagR to the C-terminus of DspB and expressed in Escherichia coli. Magnetic immobilization of purified DspB-MagR on magnetic core–shell structured Fe3O4@SiO2 nanoparticles was achieved and characterized by means of various techniques including SDS-PAGE, Fourier transform infrared spectroscopy, thermogravimetric analysis, zeta potential measurement, and scanning electron microscopy. It was evaluated the influence of temperature, pH, and storage time on the performance of immobilized DspB-MagR on Fe3O4@SiO2 nanoparticles. Removal of biofilms forming by Staphylococcus aureus and other medical sourced bacterial species was achieved by using Fe3O4@SiO2 nanoparticles loading with DspB-MagR. This work promoted potential applications of DspB and similar enzymes for medical purposes. |
| Databáze: | OpenAIRE |
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