Prostaglandin E2 enhancement of interferon-gamma production by antigen-stimulated type 1 helper T cells

Autor: Songzhu An, Li Zeng, Li Li, David Lo, Adrian Wu, Christoph W. Turck, Nabila Jabrane-Ferrat, Debra Bloom, Edward J. Goetzl
Přispěvatelé: Department of Medicine, Microbiology and Immunology (UCSF-HHMI), University of California [San Francisco] (UCSF), University of California-University of California-Rosalind Russell Medical Research Center, Center for Research in Ceramic and Composite Materials (CICECO), Universidade de Aveiro, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratory of Forest Ecology and Global Changes, School of Life Sciences
Jazyk: angličtina
Rok vydání: 1999
Předmět:
Gene Expression
Hemagglutinin Glycoproteins
Influenza Virus
MESH: Receptors
Prostaglandin E
MESH: Receptors
Prostaglandin E
EP1 Subtype
Mice
0302 clinical medicine
MESH: Animals
Prostaglandin E2
Receptor
MESH: Receptors
Prostaglandin E
EP3 Subtype
0303 health sciences
Myoglobin
MESH: Dinoprostone
MESH: Hemagglutinin Glycoproteins
Influenza Virus
MESH: Misoprostol
Receptors
Prostaglandin E
EP1 Subtype
medicine.anatomical_structure
Receptors
Prostaglandin E
EP3 Subtype
MESH: Antigens
Misoprostol
medicine.drug
Agonist
medicine.medical_specialty
MESH: Gene Expression
MESH: Myoglobin
medicine.drug_class
MESH: Interferon-gamma
T cell
Immunology
[SDV.CAN]Life Sciences [q-bio]/Cancer
Biology
Dinoprostone
03 medical and health sciences
Interferon-gamma
Immune system
Antigen
MESH: Receptors
Prostaglandin E
EP2 Subtype
Internal medicine
medicine
Animals
Receptors
Prostaglandin E
Secretion
MESH: Receptors
Prostaglandin E
EP4 Subtype
Antigens
MESH: Mice
030304 developmental biology
Interferon-gamma production
Receptors
Prostaglandin E
EP2 Subtype
Th1 Cells
Endocrinology
MESH: Th1 Cells
Receptors
Prostaglandin E
EP4 Subtype
030215 immunology
Zdroj: Cellular Immunology
Cellular Immunology, Elsevier, 1999, 194 (1), pp.21-7. ⟨10.1006/cimm.1999.1479⟩
ISSN: 0008-8749
1090-2163
DOI: 10.1006/cimm.1999.1479⟩
Popis: International audience; Prostaglandin E2 (PGE2) is a potent mediator generated in immune tissues by cyclooxygenation of arachidonic acid. PGE2 affects T cell functions through four homologous G protein-coupled receptors termed EP1R, EP2R, EP3R, and EP4R that differ in tissue distribution and signaling. Antigen-evoked secretion of interferon-gamma (IFN-gamma) by sperm whale myoglobin-specific Th1 cells of DBA/2 mouse I-Ed-restricted clones, that express EP3Rs and EP4Rs, was enhanced a maximum of 3-fold by 10(-10) to 10(-8) M PGE2 and 2.5-fold each for the EP1R/EP3R-directed agonist sulprostone (10(-8) and 10(-7) M) and for the EP4R/EP3R/EP2R agonist misoprostol (10(-9) M). Neither PGE2 nor the synthetic analogs affected secretion of IFN-gamma by PMA plus ionomycin-stimulated clones of Th1 cells. Antigen-evoked secretion of IFN-gamma by influenza hemagglutinin-specific mouse lymph node Th1 cells, that also express EP3Rs and EP4Rs, was increased a maximum of 12-fold by 10(-9) to 10(-8) M PGE2, 14-fold by 10(-9) M sulprostone, and 10-fold by 10(-9) M misoprostol. Production of IFN-gamma by either type of Th1 cell was not affected significantly by 10(-6) M PGE2 alone. The generation of IFN-gamma by antigen-stimulated Th1 cells thus is significantly enhanced by physiologically relevant concentrations of PGE2.
Databáze: OpenAIRE
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