Diagnosis of histoplasmosis by detection of the internal transcribed spacer region of fungal rRNA gene from a paraffin-embedded skin sample from a dog in Japan
| Autor: | Fukuko Kishi, Kazuko Nishimura, Yuzuru Mikami, Makoto Miyaji, Junko Ito, Katsuhiko Kamei, Tomo Inomata, Koji Yokoyama, Yachiyo Ueda, Ayako Sano, Miki Tamura |
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| Rok vydání: | 2003 |
| Předmět: |
Pathology
medicine.medical_specialty Histoplasma Molecular Sequence Data Biology Microbiology Polymerase Chain Reaction Histoplasmosis law.invention Dogs Japan law Sequence Homology Nucleic Acid Ajellomyces capsulatus DNA Ribosomal Spacer medicine Animals Dog Diseases Internal transcribed spacer Polymerase chain reaction Mycosis Skin General Veterinary Base Sequence Macrophages RNA Fungal General Medicine Ribosomal RNA medicine.disease RNA Ribosomal 5.8S Female Nested polymerase chain reaction Epizootic lymphangitis |
| Zdroj: | Veterinary microbiology. 94(3) |
| ISSN: | 0378-1135 |
| Popis: | The lesions of histoplasmosis in dogs in Japan differ from those in dogs in North America. Affected dogs in Japan have had multiple granulomatous or ulcerated foci in skin or gingiva and have not had pulmonary or gastrointestinal lesions. The present report introduces a polymerase chain reaction (PCR) diagnosis of canine histoplasmosis and the characteristic of disease in Japan. The surgically removed skin ulcerate samples from a 5-years-old female Shiba-inu native to Japan without traveling out of the country were evaluated. Tissue samples had many yeast-like organisms in the macrophages. DNA was extracted from paraffin-embedded tissue samples. A nested PCR technique was applied. The detected sequence of the internal transcribed spacer of ribosomal RNA gene had 99.7% in homology with Ajellomyces capsulatus (the teleomorph of Histoplasma capsulatum). Clinical manifestations, historical background of equine epizootic lymphangitis in Japan, and a human autochthonous case of histoplasmosis farciminosi indicated that this dog might have been infected with H. capsulatum var. farciminosum as a heteroecism. |
| Databáze: | OpenAIRE |
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