Mast Cells Contribute to Radiation-Induced Vascular Hyperpermeability
| Autor: | Dai Fukumura, Wayne L. Monsky, Dong Heui Kim, Rakesh K. Jain, Chang Geol Lee, Chang Ho Song, Kyung Ran Park |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Endothelium Biophysics Serum albumin Vascular permeability Radiation Dosage Article Permeability Capillary Permeability Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Fibrosis Skin Physiological Phenomena Internal medicine medicine Animals Radiology Nuclear Medicine and imaging Mast Cells Serum Albumin Skin Mice Inbred C3H Radiation biology Degranulation Dose-Response Relationship Radiation medicine.disease Nitric oxide synthase Vascular endothelial growth factor Dose–response relationship 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry 030220 oncology & carcinogenesis Immunology biology.protein Endothelium Vascular |
| Zdroj: | Radiation Research. 185:182-189 |
| ISSN: | 1938-5404 0033-7587 |
| DOI: | 10.1667/rr14190.1 |
| Popis: | Induction of vascular hyperpermeability is one of the early vascular responses to radiation exposure and is considered to contribute to subsequent fibrosis and tissue injuries. However, the mechanism underlying radiation-induced hyperpermeability has not yet been clearly elucidated. Here, we provide experimental evidence indicating that mast cells contribute to the increase in vascular permeability for albumin in normal mouse skin after irradiation. Vascular permeability in the skin of C3H mice increased after 2, 15 and 50 Gy irradiation, peaked at 24 h after irradiation and gradually decreased thereafter to the baseline level within 3-10 days. Both the extent and duration of hyperpermeability were dose dependent. We found significant degranulation of mast cells in the skin after 15 Gy irradiation. To further investigate the role of mast cells in the radiation-induced increase in vascular permeability, we measured vascular permeability in the skin of mast cell-deficient mice (WW(v)) and their wild-type littermates at 24 h after irradiation. Vascular permeability in WW(v) mice did not change, whereas that in wild-type mice significantly increased after irradiation. There were no appreciable changes in the total tissue levels of vascular endothelial growth factor or endothelial nitric oxide synthase after 15 Gy irradiation and there was no detectable expression of inducible nitric oxide synthase. Collectively, these results show that exposure to radiation induces vascular hyperpermeability in a dose-dependent manner and that mast cells contribute to this process. |
| Databáze: | OpenAIRE |
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