Evaluation of biochemical and serological methods to identify and clustering yeast cells of oral Candida species by CHROMagar test, SDS-PAGE and ELISA
Autor: | Flavio Cesar Almeida Tavares, José Francisco Höfling, Keila Maria Roncato Duarte, Ricardo Antunes Azevedo, Reginaldo Bruno Gonçalves, Janaina Aparecida de Oliveira Rodrigues |
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Rok vydání: | 2004 |
Předmět: |
numerical analysis
chromogenic medium (CHROMagar Candida) Enzyme-Linked Immunosorbent Assay Biology Sensitivity and Specificity Serology Microbiology Antigen Kluyveromyces marxianus Candidiasis Oral lcsh:Botany lcsh:Zoology Candida albicans Candida species Animals Cluster Analysis Humans lcsh:QL1-991 lcsh:Science Mycological Typing Techniques lcsh:QH301-705.5 Antiserum Reproducibility of Results Gel electrophoresis of proteins biology.organism_classification Isolation (microbiology) Corpus albicans Yeast lcsh:QK1-989 Culture Media protein electrophoresis lcsh:Biology (General) lcsh:Q Electrophoresis Polyacrylamide Gel Rabbits General Agricultural and Biological Sciences immunoenzimatic assay (ELISA) |
Zdroj: | Brazilian Journal of Biology, Vol 64, Iss 2, Pp 317-326 Brazilian Journal of Biology v.64 n.2 2004 Brazilian Journal of Biology Instituto Internacional de Ecologia (IIE) instacron:IIE |
ISSN: | 1519-6984 |
Popis: | The purpose of this work was to evaluate biochemical and serological methods to characterize and identify Candida species from the oral cavity. The strains used were five Candida species previously identified: C. albicans, C. guilliermondii, C. parapsilosis, C. krusei, C. tropicalis, and Kluyveromyces marxianus, as a negative control. The analyses were conducted through the SDS-PAGE associated with statistical analysis using software, chromogenic medium, and CHROMagar Candida (CA), as a differential medium for the isolation and presumptive identification of clinically important yeasts and an enzyme-linked immunoabsorbent assay (ELISA), using antisera produced against antigens from two C. albicans strains. This method enabled the screening of the three Candida species: C. albicans, C. tropicalis, and C. Krusei, with 100% of specificity. The ELISA using purified immunoglobulin G showed a high level of cross-reaction against protein extracts of Candida species. The SDS-PAGE method allowed the clustering of species-specific isolates using the Simple Matching coefficient, S SM = 1.0. The protein profile analysis by SDS-PAGE increases what is known about the taxonomic relationships among oral yeasts. This methodology showed good reproducibility and allows collection of useful information for numerical analysis on information relevant to clinical application, and epidemiological and systematical studies. |
Databáze: | OpenAIRE |
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