Effect of Intestinal Microflora on the Production of Interleukin 10 and Prostaglandin E2 in Serum and Kupffer Cells from Germfree and Conventional Mice
| Autor: | Masamichi Ikeda, Yukiko Toyama, Bunsaku Sakakibara, Tikae Katagiri, Hideo Ohira |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
prostaglandin E2 Nutrition and Dietetics business.industry Clinical Biochemistry Medicine (miscellaneous) germfree mice Single injection lipopolysacharide Proinflammatory cytokine Interleukin 10 Endocrinology In vivo Internal medicine medicine lipids (amino acids peptides and proteins) Original Article Kupffer cells Prostaglandin E2 interleukin 10 business After treatment medicine.drug |
| Zdroj: | Journal of Clinical Biochemistry and Nutrition |
| ISSN: | 1880-5086 0912-0009 |
| Popis: | To determine why germfree (GF) mice are less productivity of proinflammatory cytokines than conventional (CV) mice, we studied serum levels of interleukin 10 (IL-10) and prostaglandin E(2) (PGE(2)) in mice after treatment with lipopolyssacharide (LPS). A single injection of LPS caused an elevation of IL-10 in serum from GF, LPS-GF (germfree mice given drinking water containing LPS) and CV mice. The response was highest in serum from GF mice, and was lower in serum from LPS-GF mice compared with GF mice. Before LPS injection, serum PGE(2) was significantly higher in CV and LPS-GF mice than in GF ones. After LPS injection, a higher level of PGE(2) was maintained over 12 h in CV mice after LPS injection, while the LPS treatment reduced the level in LPS-GF mice and increased the level in GF mice. The levels of IL-10 in culture medium from Kupffer cells treated with LPS showed similar results to serum in GF and CV mice. These results suggest that high levels of IL-10 in serum from germfree mice may be partly responsible for the lower in vivo responsiveness of these proinflammatory cytokines to LPS in these mice, although PGE(2) was not responsible for the lower responsiveness of these inflammatory cytokines to LPS. |
| Databáze: | OpenAIRE |
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